Plant, Cell & Environment
Abstract
Fe and Zn are essential micronutrients for plant growth, and the interrelationship regarding their homeostasis is very complicated. In this study, we identified a FIT‐binding protein (FBP) using the yeast two‐hybrid system. The C‐terminus of FBP binds to the bHLH domain of FIT, abolishing the DNA‐binding capacity of FIT. Knockout ofFBPresults in an enhanced expression ofNASgenes and a higher nicotianamine content, and thefbpmutant exhibits tolerance to excessive Zn. Physiological analyses reveal that the mutantfbpretains a larger amount of Zn in roots and transfers a greater proportion of Fe to shoots than that in wild type under Zn‐excessive stress. AsFBPis expressed in the root stele, the negative regulation caused by sequestration of FIT is restricted to this tissue, whereas other FIT‐regulated genes, such asIRT1andFRO2, which mainly expressed in root epidermis, do not show transcriptional upregulation in thefbpmutant. As an antagonistic partner, FBP offers a new approach to spatially fine‐tune the expression of genes controlled by FIT. In conclusion, our findings provide a new insight to understand the interrelationship of Fe and Zn homeostasis in plants.
| 论文编号: | DOI:10.1111/pce.13321 |
| 论文题目: | Binding Protein Is Involved in Modulating Iron and Zinc Homeostasis in Arabidopsis |
| 英文论文题目: | Binding Protein Is Involved in Modulating Iron and Zinc Homeostasis in Arabidopsis |
| 第一作者: | Chun‐Lin Chen, Yan Cui, Man Cui, Wen‐Juan Zhou, Hui‐Lan Wu, Hong‐Qing Ling |
| 英文第一作者: | Chun‐Lin Chen, Yan Cui, Man Cui, Wen‐Juan Zhou, Hui‐Lan Wu, Hong‐Qing Ling |
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| 发表年度: | 2018-05-24 |
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| 摘要: | Fe and Zn are essential micronutrients for plant growth, and the interrelationship regarding their homeostasis is very complicated. In this study, we identified a FIT‐binding protein (FBP) using the yeast two‐hybrid system. The C‐terminus of FBP binds to the bHLH domain of FIT, abolishing the DNA‐binding capacity of FIT. Knockout ofFBPresults in an enhanced expression ofNASgenes and a higher nicotianamine content, and thefbpmutant exhibits tolerance to excessive Zn. Physiological analyses reveal that the mutantfbpretains a larger amount of Zn in roots and transfers a greater proportion of Fe to shoots than that in wild type under Zn‐excessive stress. AsFBPis expressed in the root stele, the negative regulation caused by sequestration of FIT is restricted to this tissue, whereas other FIT‐regulated genes, such asIRT1andFRO2, which mainly expressed in root epidermis, do not show transcriptional upregulation in thefbpmutant. As an antagonistic partner, FBP offers a new approach to spatially fine‐tune the expression of genes controlled by FIT. In conclusion, our findings provide a new insight to understand the interrelationship of Fe and Zn homeostasis in plants. |
| 英文摘要: | Fe and Zn are essential micronutrients for plant growth, and the interrelationship regarding their homeostasis is very complicated. In this study, we identified a FIT‐binding protein (FBP) using the yeast two‐hybrid system. The C‐terminus of FBP binds to the bHLH domain of FIT, abolishing the DNA‐binding capacity of FIT. Knockout ofFBPresults in an enhanced expression ofNASgenes and a higher nicotianamine content, and thefbpmutant exhibits tolerance to excessive Zn. Physiological analyses reveal that the mutantfbpretains a larger amount of Zn in roots and transfers a greater proportion of Fe to shoots than that in wild type under Zn‐excessive stress. AsFBPis expressed in the root stele, the negative regulation caused by sequestration of FIT is restricted to this tissue, whereas other FIT‐regulated genes, such asIRT1andFRO2, which mainly expressed in root epidermis, do not show transcriptional upregulation in thefbpmutant. As an antagonistic partner, FBP offers a new approach to spatially fine‐tune the expression of genes controlled by FIT. In conclusion, our findings provide a new insight to understand the interrelationship of Fe and Zn homeostasis in plants. |
| 刊物名称: | Plant, Cell & Environment |
| 英文刊物名称: | Plant, Cell & Environment |
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| 其它备注: | Chun‐Lin Chen, Yan Cui, Man Cui, Wen‐Juan Zhou, Hui‐Lan Wu, Hong‐Qing Ling. A FIT‐Binding Protein Is Involved in Modulating Iron and Zinc Homeostasis in Arabidopsis. Plant, Cell & Environment. DOI:10.1111/pce.13321 |
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