Plant Physiology
Abstract
Ribosome biogenesis is crucial for plant growth and environmental acclimation. Processing of ribosomal RNAs (rRNAs) is an essential step in ribosome biogenesis and begins with transcription of the rDNA. The resulting pre-rRNA transcript undergoes systematic processing, where multiple endonucleolytic and exonucleolytic cleavages remove the external and internal transcribed spacers (ETS and ITS). The processing sites and pathways for pre-rRNA processing have been deciphered in Saccharomyces cerevisiae and, to some extent, in Xenopus, mammalian cells, and Arabidopsis thaliana. However, the processing sites and pathways remain largely unknown in crops, particularly in monocots such as rice (Oryza sativa L.), one of the most important food resources in the world. Here, we identified the rRNA precursors produced during rRNA biogenesis and the critical endonucleolytic cleavage sites in the transcribed spacer regions of pre-rRNAs in rice. We further found that two pre-rRNA processing pathways, distinguished by the order of 5′ ETS removal and ITS1 cleavage, co-exist in vivo. Moreover, exposing rice to chilling stress resulted in the inhibition of rRNA biogenesis mainly at the pre-rRNA processing level, suggesting that these energy-intensive processes may be reduced to increase acclimation and survival at lower temperatures. Overall, our study identified the pre-rRNA processing pathway in rice and showed that ribosome biogenesis is quickly inhibited by low temperatures, which may shed light on the link between ribosome biogenesis and environmental acclimation in crop plants.
| 论文编号: | DOI:10.1104/pp.17.01714 |
| 论文题目: | Ribosomal RNA Biogenesis and its Response to Chilling Stress in Oryza sativa L |
| 英文论文题目: | Ribosomal RNA Biogenesis and its Response to Chilling Stress in Oryza sativa L |
| 第一作者: | Runlai Hang, Zhen Wang, Xian Deng, Chun yan Liu, Bin Yan, Chao Yang, Xianwei Song, Beixin Mo, XiaoFeng Cao |
| 英文第一作者: | Runlai Hang, Zhen Wang, Xian Deng, Chun yan Liu, Bin Yan, Chao Yang, Xianwei Song, Beixin Mo, XiaoFeng Cao |
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| 发表年度: | 2018-03-21 |
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| 摘要: | Ribosome biogenesis is crucial for plant growth and environmental acclimation. Processing of ribosomal RNAs (rRNAs) is an essential step in ribosome biogenesis and begins with transcription of the rDNA. The resulting pre-rRNA transcript undergoes systematic processing, where multiple endonucleolytic and exonucleolytic cleavages remove the external and internal transcribed spacers (ETS and ITS). The processing sites and pathways for pre-rRNA processing have been deciphered in Saccharomyces cerevisiae and, to some extent, in Xenopus, mammalian cells, and Arabidopsis thaliana. However, the processing sites and pathways remain largely unknown in crops, particularly in monocots such as rice (Oryza sativa L.), one of the most important food resources in the world. Here, we identified the rRNA precursors produced during rRNA biogenesis and the critical endonucleolytic cleavage sites in the transcribed spacer regions of pre-rRNAs in rice. We further found that two pre-rRNA processing pathways, distinguished by the order of 5′ ETS removal and ITS1 cleavage, co-exist in vivo. Moreover, exposing rice to chilling stress resulted in the inhibition of rRNA biogenesis mainly at the pre-rRNA processing level, suggesting that these energy-intensive processes may be reduced to increase acclimation and survival at lower temperatures. Overall, our study identified the pre-rRNA processing pathway in rice and showed that ribosome biogenesis is quickly inhibited by low temperatures, which may shed light on the link between ribosome biogenesis and environmental acclimation in crop plants. |
| 英文摘要: | Ribosome biogenesis is crucial for plant growth and environmental acclimation. Processing of ribosomal RNAs (rRNAs) is an essential step in ribosome biogenesis and begins with transcription of the rDNA. The resulting pre-rRNA transcript undergoes systematic processing, where multiple endonucleolytic and exonucleolytic cleavages remove the external and internal transcribed spacers (ETS and ITS). The processing sites and pathways for pre-rRNA processing have been deciphered in Saccharomyces cerevisiae and, to some extent, in Xenopus, mammalian cells, and Arabidopsis thaliana. However, the processing sites and pathways remain largely unknown in crops, particularly in monocots such as rice (Oryza sativa L.), one of the most important food resources in the world. Here, we identified the rRNA precursors produced during rRNA biogenesis and the critical endonucleolytic cleavage sites in the transcribed spacer regions of pre-rRNAs in rice. We further found that two pre-rRNA processing pathways, distinguished by the order of 5′ ETS removal and ITS1 cleavage, co-exist in vivo. Moreover, exposing rice to chilling stress resulted in the inhibition of rRNA biogenesis mainly at the pre-rRNA processing level, suggesting that these energy-intensive processes may be reduced to increase acclimation and survival at lower temperatures. Overall, our study identified the pre-rRNA processing pathway in rice and showed that ribosome biogenesis is quickly inhibited by low temperatures, which may shed light on the link between ribosome biogenesis and environmental acclimation in crop plants. |
| 刊物名称: | Plant Physiology |
| 英文刊物名称: | Plant Physiology |
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| 其它备注: | Runlai Hang, Zhen Wang, Xian Deng, Chun yan Liu, Bin Yan, Chao Yang, Xianwei Song, Beixin Mo, XiaoFeng Cao. Ribosomal RNA Biogenesis and its Response to Chilling Stress in Oryza sativa L. Plant Physiology. DOI:10.1104/pp.17.01714 |
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