中文关键词:谷胱甘肽含量测定纳米酶羧基化石墨烯 英文关键词:glutathioneassaynanoparticle enzymecarboxylated graphene oxide 基金项目:国家自然科学基金项目（No. 81573834）、民族医药教育部重点实验室（中央民族大学）自主课题（No. KLEM-ZZ201808）和中央民族大学青年学术团队引领计划（2019QNYL26）资助。

作者	单位	E-mail
董旭	中央民族大学药学院	dongxu_pharmacy@163.com
郭华	中央民族大学药学院
闾春林	中央民族大学药学院
申刚义	中央民族大学药学院	sgy@iccas.ac.cn

摘要点击次数:1139 全文下载次数:0 中文摘要: 利用羧基化氧化石墨烯(GO-COOH)的纳米酶效应,建立了一种谷胱甘肽制剂比色法含量测定方法。对反应时间、pH、H₂O₂浓度进行了优化。在最佳条件下,谷胱甘肽标准品检测的线性范围为1-50 μmol /L。将该方法用于谷胱甘肽片的含量测定,并采用液相色谱法对测定结果进行了验证。结果显示谷胱甘肽片剂测定的相对误差为1.8%。该方法简单、直观,可用于谷胱甘肽制剂含量的准确测定。 英文摘要: A new method for the determination of glutathione content was established by using the nanoenzyme effect of carboxylated graphene oxide (GO-COOH). The reaction time, pH, and H2O2 concentration were optimized. Under optimal conditions, the linear range of glutathione (GSH) standards was 1-50 μmol / L. The method was applied to the determination of practical sample, and verified by high performance liquid chromatography. The results showed that the content of glutathione tablets was easily and accurately measured with the relative error of 1.8%, which indicated that the method is simple and intuitive, and can accurately measure the content of glutathione tablet. 查看全文查看/发表评论下载PDF阅读器 相关附件：版权转让声明书 -->