方晓红,1968年8月出生于湖南省。1990年毕业于武汉大学化学系,获学士学位。1993年和1996年分别于北京大学化学系获硕士学位和博士学位。1997年4月至1998年3月在加拿大滑铁卢大学化学系做博士后。1998年4月至2001年12月先后在美国佛罗里达大学化学系、生物技术中心及生物/纳米技术交叉研究中心做博士后、助理研究员。2001年4月入选中科院百人计划,2001年12月回国工作,任中国科学院化学研究所分子纳米结构与纳米技术重点实验室研究员,博士生导师。
教授,博士生导师
Tel/Fax: 62650024
E-Mail: xfang@iccas.ac.cn
研究方向
1.生物单分子荧光成像及单分子行为研究
2.蛋白质高灵敏度的实时检测
3.纳米生物传感器
4.DNA/蛋白质相互作用的研究
获得奖励
1.中科院引进国外杰出人才(百人计划)
2.国家自然科学基金委杰出青年科学基金
3.中国化学会青年化学奖
代表性研究论文
1. Signaling Aptamer/Protein Binding by a Molecular Light-Switch Complex. Jiang Y. X. Fang X Bai C. Anal. Chem. in press 2004.
2. Study of the Single Molecular Interaction of the Transcription Factor ZmDREB1A with its DNA Responsive Element by Atomic Force Microscopy.Jiang Y. X. Feng Q. Fang X Bai C. Nucl.Acid.Res. 2004,32 e101.
3. Specific Interaction of the Transcription Factor and the DNA Element Investigated by Atomic Force Microscopy.QIN Feng JIANG Yaxin MA Xinyong CHEN Feng FANG Xiaohong BAI Chunli & LI Yiqin. Chinese Science Bulletin. 2004 49 1376-1380.4.
4. Delaying Photobleaching and Recovering Luminescence of a DNA Molecular Light Switch in DNA Analysis Ling L. Jiang Y. Wang C. Fang X. Bai]C. Chen. D. Anal. Biochem. 2004 329 334-336
5. Progress in the Study and Application of Molecular Beacon Jiang Y. X. Fang X. Wan L. J. Bai C. L Chinese Anal. Chem. 2004 32 668-672.
6. Specific Aptamer - Protein Interaction Studied by Atomic Force Microscopy. Jiang Y. X. Zhu C. F. Ling L.S. Wan L. J. Fang X. Bai C. L. Anal. Chem. 2003 75(9): 2112-2116
7. Study of Citrate Adsorbed on the Au(111) Surface by Scanning Probe Microscopy. Lin Y. Pan G. B. Su G. J. Fang X. H. Wan L. J. Bai C. L. Langmuir 2003 19 10000-1003
8. Synthetic DNA Aptamers to Detect Protein Molecular Variants in a High-Throughput Fluorescence Quenching Assay,Fang X. Sen A. Vicence M. and Tan W.,ChemBioChem,2003. 4(9): 829-834
9. Monitoring Molecular Beacon DNA Probe Hybridization at the Single-Molecule Level,Yoa G Fang X. Yokota H. A. Yanagida T. and Tan W,Chemistry - A European Journal,2003 9: 5686-5692
10.AFM and STM study of B-amyloid aggregation on graphite. Wang Z. Zhou C. Fang X. Wan L. Wang C. Bai C. Ultramicroscopy 2003,97,73-79.
11.Directly Observe the DNA Multimolecule Condensation with Fluorescence Microscopy. Ling L;. Fang X. Wang C, Wan L. Chen D.; Bai C; Chemistry Letter 2003,32(1);80-81.
12 Progress in the Study of Aptamer Wang J. Jiang Y. X. Fang X.. Bai C. L Chinese Physics,2003 32(11) 662-665
13. Molecular Beacons: "Lights up" Probes For Living Cell Study. Fang X.; Mi Y.; Li J. Beck T Schuster S; Tan W. Cell Biochemistry and Biophysics 2002 37(2):71-81.
14. Molecular aptamer beacons for real-time protein recognition. Li J. Fang X.; Tan W. Biochem Biophys Res Commun. 2002 Mar 22;292(1):31-40.
15. Molecular Beacons: Novel probes for detection and quantification of biologically significant molecules Perlette J.; Li J; Fang X.; Schuster S; Tan W.; Reviews in Analytical Chemistry 2002 21 (1): 1-14.
16.Novel Fluorescent Molecular Beacon DNA Probes for biomolecular Recognition ,Wang K. Li J. Fang X. Schuster S; Skelly S. Lou H. Li J. J. Beck T. Hogrefe R. Tan W. Biomedical Photonics Handbook (Ed. Tuan Vo-Dinh) Academic Press 2002, 57(1)-57(23).
17. Molecular Aptamer for Real-time Oncoprotein PDGF Monitoring by Fluorescence Anisotropy Fang X. Cao Z. Beck T. and Tan W. Anal. Chem. 74 (23) 2001 5752-5757.
18. Molecular Beacons: A Novel Fluorescent Probe for Biomolecule Recognition;Fang X.; Li J.; K. Wang Tan W. Anal. Chem. 2000 72(23): 747A-753A
19. Using Molecular Beacons to Probe the Interactions Between Lactic Dehydrogenase and Single Strand DNA;Fang X.; Li J.; Tan W. Anal. Chem.,2000 72(14): 3280-3285
20. Molecular Beacons: Detecting Protein-Nucleic Acid Interactions;Li J.; Fang X.; Tan W.; Angewandte Chemie International Edition 2000 39: 1049-1052;
21. Molecular Beacons: A novel DNA Probe for Nucleic Acids and Protein Studies;Tan W.; Fang X.; Li J.; Liu X.; Chemistry A European Journal 2000,6:1087-1092.
22. Single Molecules Imaging and Interaction Study Using Evanescent Wave Excitation Fang X.; Tan W. American Biotechnology Laboratory 2000 18(5) : 64-65
23. Design a Novel Molecular Beacon for Immobilized DNA Hybridization;Fang X.;Liu X.; Schuster S.; Tan W. J. Amer. Chem. Soci. 1999,121:2921-2922
24. Imaging Single Fluorescent Molecules at the Interface of an Optical Fiber Probe by Evanescent Wave Excitation;Fang X.; Tan W.; Anal. Chem. 1999 71: 3101-3105主要贡献
从事生物物理生物化学分析,发展在单分子和单细胞水平上的高灵敏度的生物医学分析新方法,应用扫描探针显微技术对生物分子进行表征,操纵和研究生物分子的相互作用。在研制用于DNA、蛋白质实时检测的新型荧光探针,单分子光学成像和在单分子水平上研究生物分子相互作用等方面取得了一些较高水平的成绩:
(1)发展新型DNA核酸识体和分子信标荧光探针并用于DNA、蛋白质相互作用的研究和蛋白质的检测;
(2)首次利用光纤探针进行单分子荧光成像;
(3)利用扫描探针显微技术研究DNA/蛋白质单分子相互作用力。在JACS,Anal. Chem.,Angewandte Chemie, Nucl. Acid. Res.等杂志上发表论文50多篇。