基本信息
杨开广男博导中国科学院大连化学物理研究所
电子邮件： yangkaiguang@dicp.ac.cn
通信地址： 辽宁省大连市中山路457号 大连化物所1810组
邮政编码： 116023
部门/实验室：研究生部
研究领域

蛋白质组学新技术新方法；蛋白质富集与分离材料；分子印迹



教育背景2007-09--2009-02Lund University联合培养博士生
2003-09--2009-06四川大学博士
1999-09--2003-06成都理工大学学士


专利与奖励



专利：
申请发明专利144项，其中已授权55项。


获奖：
大连化物所冠名奖“青年优秀奖”,一等奖,研究所（学校）,2016


代表性专利成果：
（ 1 ） 一种聚乙二醇偶联结合超滤离心分离富集糖肽的方法, 发明, 2011, 专利号: 1.X
（ 2 ） 一种亲水性金属离子固定化亲和纳米磁球的制备方法, 发明, 2011, 专利号: 7.0
（ 3 ） 多种蛋白质肽段同时印迹聚合物微球材料及其制备和应用, 发明, 2016, 专利号: 4.7
（ 4 ） 基于RAFT策略蛋白质表面分子印迹材料及制备和应用, 发明, 2016, 专利号: 3.6
（ 5 ） 一种生物样品纯化的装置及其应用, 发明, 2017, 专利号: 6.2
（ 6 ） 一种聚合物纳米微球的制备方法, 发明, 2017, 专利号: 8.4
（ 7 ） 一种核壳结构的聚合物微球及其制备和应用, 发明, 2018, 专利号: 9.1
（ 8 ） 一种有机无机杂化微球颗粒及其制备和应用, 发明, 2018, 专利号: 4.4
（ 9 ） 基于质谱的氧连接氮乙酰葡糖胺修饰糖蛋白质的分析方法, 发明, 2019, 专利号: 6.8
（ 10 ） 赖氨酸氮连接磷酸化翻译后修饰富集和鉴定的方法, 发明, 2019, 专利号: 0.6








出版信息




申请人已在Advanced Materials, Anal. Chem.，Chem. Commun.，ACS Appl. Mater.Interfaces，Nanoscale，Macromolecules，Sci. Rep.等杂志发表SCI 文章80 余篇，他引1500 余次，H 因子25













发表论文（1）Cell-imprinted polydimethylsiloxane for the selective cell adhesion,Chin. Chem. Lett.,2019,第1作者
（2）Artificial Antibody with Site-Enhanced Multivalent Aptamers for Specific Capture of Circulating Tumor Cells,Anal. Chem.,2019,第1作者
（3）Poly(ether sulfone) nanoparticles and controllably modified nanoparticles obtained through temperature-dependent cryogelation,J. Appl. Polym. Sci.,2019,第1作者
（4）Proteomics investigation of the changes in serum proteins after high- and low-flux hemodialysis,Renal Failure,2018,第1作者
（5）3-Carboxybenzoboroxole Functionalized Polyethylenimine Modified Magnetic Graphene Oxide Nanocomposites for Human Plasma Glycoproteins Enrichment under Physiological Conditions,Anal. Chem.,2018,第7作者
（6）"Thiol-ene" grafting of silica particles with three-dimensional branched copolymer for HILIC/cation-exchange chromatographic separation and N-glycopeptide enrichment,Anal. Bioanal. Chem.,2018,第4作者
（7）Surface sieving coordinated IMAC material for purification of His-tagged proteins,Analytica Chimica ACTA,2018,第1作者
（8）Proteomics Investigations into Serum Proteins Adsorbed by High-Flux and Low-Flux Dialysis Membranes,Proteomics-Clinical Application,2017,第1作者
（9）Aptamer-immobilized open tubular capillary column to capture circulating tumor cells for proteome analysis,Talanta,2017,第1作者
（10）Proteomic study provides new clues for complications of hemodialysis caused by dialysis membrane,Science Bulletin,2017,第1作者
（11）Multiepitope Templates Imprinted Particles for the Simultaneous Capture of Various Target Proteins,Anal. Chem.,2016,第1作者
（12）Thermoresponsive Epitope Surface-Imprinted Nanoparticles for Specific Capture and Release of Target Protein from Human Plasma,ACS Appl. Mater. Interfaces,2016,第1作者
（13）Epitope imprinting enhanced IMAC (EI-IMAC) for highly selective purification of His-tagged protein,J. Mater. Chem. B,2016,第1作者
（14）Boronic Acid-Functionalized Particles with Flexible Three-Dimensional Polymer Branch for Highly Specific Recognition of Glycoproteins,ACS Appl. Mater. Interfaces,2016,第1作者
（15）Preparation of surface imprinted core-shell particles via a metal chelating strategy: specific recognition of porcine serum albumin,Microchim. Acta,2016,第2作者
（16）Clickable Periodic Mesoporous Organosilica Monolith for Highly Efficient Capillary Chromatographic Separation.,Anal. Chem.,2016,第3作者
（17）Surface-imprinted nanoparticles prepared with a His-tag-anchored epitope as the template,Anal. Chem.,2015,第2作者
（18）An efficient approach to prepare boronate core-shell polymer nanoparticles for glycoprotein recognition via combined distillation precipitation polymerization and RAFT media precipitation polymerization,Chem Commun,2015,第2作者
（19）Dandelion-like core–shell silica microspheres with hierarchical pores,RSC Adv.,2015,第2作者
（20）Glycoprotein recognition by water-compatible core-shell polymeric submicron particles,J. Mat. Chem. B,2015,第3作者
（21）Polyethyleneimine-modified graphene oxide nanocomposites for effective protein functionalization,Nanoscale,2015,第3作者
（22）Epitope imprinted polyethersulfone beads by self-assembly for target protein capture from the plasma proteome,Chem Commun,2014,第1作者
（23）Monodisperse Boronate Polymeric Particles Synthesized by a Precipitation Polymerization Strategy: Particle Formation and Glycoprotein Response from the Standpoint of the Flory-Huggins Model,ACS Appl. Mater. Interfaces,2014,第3作者
（24）Surface Protein Imprinted Core–Shell Particles for High Selective Lysozyme Recognition Prepared by Reversible Addition–Fragmentation Chain Transfer Strategy,ACS Appl. Mater. Interfaces,2014,第1作者
（25）Preparation of protein imprinted materials by hierarchical imprinting techniques and application in selective depletion of albumin from human serum,Sci Rep,2014,第4作者
（26）Transferrin recognition based on a protein imprinted material prepared by hierarchical imprinting technique,Microchim. Acta,2013,第2作者
（27）Hydrophilic immobilized trypsin reactor with magnetic graphene oxide as support for high efficient proteome digestion,J. Chromatogr. A,2012,第2作者
（28）Protein Imprinted Materials: Rational Design, Application and Challenges,Analytical and Bioanalytical Chemistry,2012,第1作者
（29）Preparing a metal-ion chelated immobilized enzyme reactor based on the polyacrylamide monolith grafted with polyethylenimine for a facile regeneration and high throughput tryptic digestion in proteomics,Analytical and Bioanalytical Chemistry,2012,通讯作者
（30）Boronic acid functionalized core-shell polymer nanoparticles for selective enrichment of glycopeptides,Chemistry - A European Journal,2012,第3作者
（31）A novel organic-inorganic hybrid monolith for trypsin immobilization,Sci. China-Life Sci.,2011,第3作者
（32） Urea free and more efficient sample preparation method for mass spectrometry based protein identification via combining the formic acid-assisted chemical cleavage and trypsin digestion,Talanta,2011,通讯作者
（33）Preparation of a new type of affinity materials combining metal coordination with molecular imprinting,Chemical Communications,2011,第2作者
（34）Macroporous molecularly imprinted monolithic polymer columns for protein recognition by liquid chromatography,Journal of separation science,2010,第3作者
（35）Self-Assembly Molecularly Imprinted Nanofiber for 4-HA Recognition,Analytical Letters,2010,第3作者
（36）One-Pot Synthesis of Hydrophilic Molecularly Imprinted Nanoparticles,Macromolecules,2009,第1作者
（37）Molecularly imprinted porous polysulfone particles for the binding and recognition of bisphenol A,Desalination,2009,第1作者
（38）BPA transfer rate increase using molecular imprinted polyethersulfone hollow fiber membrane.,J. Membr. Sci.,2008,第4作者
（39）Effect of the template molecules and nonsolvent additives on the recognition property of molecular imprinted polyethersulforte particles.,J. Appl. Polym. Sci.,2008,第4作者
（40）Preparation and selective binding characterization of bisphenol a imprinted polyethersulfone particles,J. Appl. Polym. Sci.,2007,第1作者
（41）DNA-loaded porous polyethersulfone particles for environmental applications I. Preparation,J. Appl. Polym. Sci.,2005,第3作者
（42）DNA-loaded porous polyethersulfone particles for environmental applications II. Utilization,J. Appl. Polym. Sci.,2005,第2作者
（43）Characterization of DNA-loaded porous polyethersulfone particles prepared by phase inversion technique.,Colloid Journal,2005,第2作者
（44）Molecularly imprinted polyethersulfone microspheres for the binding and recognition of bisphenol A,Anal. Chim. Acta,2005,第1作者
（45）Preparation of DNA-encapsulated polyethersulfone hollow microspheres for organic compounds and heavy metal ions removal,Desalination,2005,第1作者
（46）DNA-modified porous polysulfone microspheres for selective removal of endocrine disruptors,Chemical Journal of Chinese Universities-Chinese,2005,第2作者
（47）Preparation of DNA hybrid polyethersulfone microspheres for endocrine disruptor removal,Key Engineering Materials,2005,第2作者
（48）DNA-immobilized porous polysulfone beads for organic compounds and heavy metal ion removal.,Desalination,2004,第2作者
（49）Preparation of porous polysulfone beads for selective removal of endocrine disruptors,Separation and Purification Technology,2004,第3作者

发表著作（1）蛋白质组学研究-概念、技术及应用,Proteome Research--Concepts, Technology and Application,科学出版社,2010-05,第5作者


科研活动






科研项目（ 1 ） 用于血浆高丰度蛋白质选择性去除的多抗原决定基表面印迹磁球的研究, 主持,国家级,2011-01--2013-12
（ 2 ） 基于相反转自组装技术的蛋白质 C-末端肽印迹材料制备及机理研究, 主持,国家级,2014-01--2017-12
（ 3 ） 针对细胞识别的人工抗体材料制备及其在循环肿瘤细胞捕获中的应用, 主持,国家级,2016-01--2019-12
（ 4 ） 蛋白质组富集分离与修饰鉴定的关键技术研究与开发, 参与,国家级,2012-01--2015-12
（ 5 ） 质谱鉴定新方法研究, 参与,国家级,2016-07--2019-12
（ 6 ） 中国科学院青年创新促进会第七批会员资助, 主持,部委级,2017-01--2020-12
（ 7 ） 基于交联剂跨膜靶向转运载体的细胞内蛋白质复合物原位靶向分析方法, 主持,国家级,2019-01--2022-12