基本信息
王曼丽女博导中国科学院武汉病毒研究所
电子邮件： Wangml@wh.iov.cn
通信地址： 湖北省武汉市武昌小洪山中区44号
邮政编码： 430071


研究领域?长期从事昆虫病毒和虫媒布尼亚病毒的感染机理研究，特别是在病毒入侵机制和抗病毒药物研究中取得了一系列创新性的研究成果。截至目前，共发表SCI研究论文60余篇。其中，第一作者和通讯作者SCI论文30余篇，包括Cell Research (1)、Small (1)、PhilosophicalTransactionsB (1)、Journal of Virology (9)等。近期的研究重点为病毒入侵细胞的分子机制、病毒与媒介/宿主昆虫免疫系统的互作机制、病毒的致病机制和抗病毒药物。



招生信息重要新发病毒的感染机理、致病机制和抗病毒药物。



招生专业071005-微生物学

招生方向分子病毒学，生物工程技术


教育背景2002-09--2008-09中国科学院武汉病毒研究所博士学位
1998-09--2002-07武汉工业学院学士学位

学历研究生学历

学位2009年获微生物学博士学位

工作经历
工作简历2011-09~现在,中国科学院武汉病毒研究所,副研究员
2008-10~2011-08,中国科学院武汉病毒研究所,助理研究员
2007-03~2007-10,荷兰wageningen大学,访问

社会兼职

教授课程文献阅读
《分子病毒学》第四讲
《分子病毒学》第五讲


专利与奖励?


奖励信息（1） 病毒所青年年度人物, 研究所（学校）, 2015
（2） 中国科学院卢嘉锡青年人才奖, , 院级, 2012
（3） 中国科学院优秀博士学位论文, , 院级, 2010
（4） 湖北省优秀博士学位论文, , 省级, 2010

专利成果（ 1 ） 用于提高杆状病毒对哺乳动物细胞, 发明, 2011, 第 3 作者, 专利号: 7.2
（ 2 ） 表达gp64基因的新型基因工程重组, 发明, 2011, 第 2 作者, 专利号: 05


出版信息
发表论文（1）Remdesivir and chloroquine effectively inhibit the recently emerged novel coronavirus (2019-nCoV) in vitro.,Cell Research,2020-03,第1作者
（2）Hydroxychloroquine, a less toxic derivative of chloroquine, is effective in inhibiting SARS-CoV-2 infection in vitro,Cell Discovery,2020-03,通讯作者
（3）Host AAA+ ATPase TER94 Plays Critical Roles in Building the Baculovirus Viral Replication Factory and Virion Morphogenesis.,Journal of Virology,2020-02,通讯作者
（4）Functional Characterization of the Group I Alphabaculovirus Specific Gene ac73.,Virologica Sinica,2019-12,通讯作者
（5）Baculovirus ODV-E66 degrades larval peritrophic membrane to facilitate baculovirus oral infection.,Virology,2019-11,通讯作者
（6）Genome Analysis of a Novel Clade II.b Alphabaculovirus Obtained from Artaxa digramma.,Viruses,2019-10,通讯作者
（7）Host factor heat-shock protein 90 contributes to baculovirus budded virus morphogenesis via facilitating nuclear actin polymerization.,Virology,2019-09,通讯作者
（8）The cysteine-rich region of a baculovirus VP91 protein contributes to the morphogenesis of occlusion bodies.,Virology,2019-09,通讯作者
（9）Improving Baculovirus Transduction of Mammalian Cells by Incorporation of Thogotovirus Glycoproteins.,Virologica Sinica,2019-08,通讯作者
（10）Advances in Molecular Biology of Baculoviruses.,Curr Issues Mol Biol.,2019-06,第1作者
（11）The major hurdle for effective baculovirus transduction into mammalian cells is passing early endosomes.,Journal of Virology,2019,通讯作者
（12）Cross-talking between baculoviruses and host insects towards a successful infection.,Philos Trans R Soc Lond B Biol Sci.,2019,通讯作者
（13）Quantitative Proteomic Analysis Reveals Unfolded-Protein Response Involved in Severe Fever with Thrombocytopenia Syndrome Virus Infection.,Journal of Virology,2019,通讯作者
（14）Baculovirus Per Os Infectivity Factor Complex: Components and Assembly.,Journal of Virology,2019,通讯作者
（15）Single-Particle Tracking Reveals the Sequential Entry Process of the Bunyavirus Severe Fever with Thrombocytopenia Syndrome Virus.,Small,2019,通讯作者
（16）Genome Characteristics of the Cyclophragma Undans Nucleopolyhedrovirus: A Distinct Species in Group I of Alphabaculovirus.,Virologica Sinica,2018,通讯作者
（17）The Functional Oligomeric State of Tegument Protein GP41 Is Essential for Baculovirus Budded Virion and Occlusion-Derived Virion Assembly.,Journal of Virology,2018,通讯作者
（18）HearNPV Pseudotyped with PIF1, 2, and 3 from MabrNPV: Infectivity and Complex Stability.,Virologica Sinica,2018,通讯作者
（19）Genome analysis of a novel Group I alphabaculovirus obtained from Oxyplax ochracea.,PLoS One,2018,通讯作者
（20）Three Conserved Regions in Baculovirus Sulfhydryl Oxidase P33 Are Critical for Enzymatic Activity and Function.,Journal of Virology,2018,第3作者
（21）Inhibition of melanization by serpin-5 and serpin-9 promotes baculovirus infection in cotton bollworm Helicoverpa armigera.,PLoS Pathogens,2017,第3作者
（22）Per os infectivity factors: a complicated and evolutionarily conserved entry machinery of baculovirus.,Science China Life Science,2017,通讯作者
（23）Deltabaculoviruses encode a functional type I budded virus envelope fusion protein.,J Gen Virol,2017,第1作者
（24）Construction and Rescue of a Functional Synthetic Baculovirus.,ACS Synthetic Biology,2017,第2作者
（25）Characterization of the viral fibroblast growth factor homolog of Helicoverpa armigera single nucleopolyhedrovirus.,Virologica Sinica,2016,通讯作者
（26）Mutational and functional analysis of N-linked glycosylation of envelope fusion protein F of Helicoverpa armigera nucleopolyhedrovirus.,J Gen Virol,2016,第2作者
（27）Characterization of two monoclonal antibodies, 38F10 and 44D11, against the major envelope fusion protein of Helicoverpa armigera nucleopolyhedrovirus,Virologica Sinica,2016,通讯作者
（28）The Host Specificities of Baculovirus per os Infectivity Factors.,PLoS One,2016,通讯作者
（29）The FP25K Acts as a Negative Factor for the Infectivity of AcMNPV Budded Virus.,Plos One,2015,第2作者
（30）Glycoprotein E of the Japanese encephalitis virus forms virus-like particles and induces syncytia when expressed by a baculovirus.,Journal of General Virolog,2015,第3作者
（31）Identification and functional analysis of inter-subunit disulfide bonds of the F protein of Helicoverpa armigera nucleopolyhedrovirus.,Journal of General Virology,2014,第2作者
（32）Viral suppression of innate immunity via spatial isolation of TBK1/IKKε from mitochondrial antiviral platform.,Journal of Molecular Cell Biology,2014,第2作者
（33）Unraveling the entry mechanism of baculoviruses and its evolutionary implications.,Journal of Virology,2014,第1作者
（34）ORF85 of HearNPV encodes the per os infectivity factor 4 (PIF4) and is essential for the formation of the PIF complex.,Virology,2012,第2作者
（35）Functional studies of per os infectivity factor 3 of Helicoverpa armigera nucleopolyhedrovirus.,Journal of General Virology,2012,第2作者
（36）Partial functional rescue of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus infectivity by replacement of F protein with GP64 from Autographa californica multicapsid nucleopolyhedrovirus.,Journal of Virology,2010,第1作者
（37）Specificity of baculovirus P6.9 basic DNA-binding proteins and critical role of the C terminus in virion formation.,Journal of Virology,2010,第1作者
（38）Autographa californica multicapsid nucleopolyhedrovirus efficiently infects Sf9 cells and transduces mammalian cells via direct fusion with the plasma membrane at low pH.,Journal of Virology,2010,第2作者
（39）The F-like protein Ac23 enhances the infectivity of the budded virus of gp64-null Autographa californica multinucleocapsid nucleopolyhedrovirus pseudotyped with baculovirus envelope fusion protein F.,Journal of Virology,2008,第1作者
（40）The F protein of Helicoverpa armigera single nucleopolyhedrovirus can be substituted functionally with its homologue from Spodoptera exigua multiple nucleopolyhedrovirus.,Journal of General Virology,2008,第1作者
（41）Mutagenesis and nuclear magnetic resonance analyses of the fusion peptide of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus F protein.,Journal of Virology,2008,第3作者
（42）The F protein of Helicoverpa armigera single nucleopolyhedrovirus can be substituted functionally with its homologue from Spodoptera exigua multiple nucleopolyhedrovirus.,Journal of Virology,2008,第2作者

发表著作《Insect Molecular Virology》Caister Academic Press；2019.01；参编第九章

科研活动
科研项目（ 1 ） 杆状病毒口服感染分子机制的研究, 参与,国家级,2012-01--2016-12
（ 2 ） 重要病毒转录复制蛋白复合体的结构功能研究, 主持,国家级,2013-01--2017-12
（ 3 ） 宿主细胞因子Integrin在杆状病毒入侵哺乳动物细胞中的功能及其作, 主持,国家级,2014-01--2017-12
（ 4 ） 中科院青年创新促进会, 主持,国家级,2013-01--2016-12
（ 5 ） 关键宿主因子Hsp90介导的昆虫细胞actin核内聚合在杆状病毒BV形成中的作用机制, 主持,国家级,2016-01--2019-12
（ 6 ） 杆状病毒感染昆虫中肠的作用机理研究, 参与,部委级,2016-08--2020-12
（ 7 ） P4实验室高端用户培育计划, 主持,部委级,2018-08--2019-12
（ 8 ） 2019-nCoV药物筛选与评价研究, 参与,国家级,2020-01--2020-12
（ 9 ） 定向项目, 参与,国家级,2019-01--2023-12

参与会议（1）Functional Rescue of an F-null HearNPV by two Naturally Non-functional Envelope Proteins Disclose the Evolutionary Track of Baculoviridae第29届美国病毒学会年会Manli Wang, Feifei Yin, Ying Tan, Fei Deng, Just M Vlak2, Johannes A. Jehle, Zhihong Hu and Hualin Wang2010-07-17
（2）The F-like protein of group I NPVs enhances the production and infectivity of the budded virus of gp64-null AcMNPV pseudotyped with the envelope fusion protein F of group II NPVs第41届国际无脊椎动物病理学年会Manli Wang, Ying Tan, Feifei Yin, Fei Deng, Zhihong Hu, Just M Vlak and Hualin Wang2008-08-03


合作情况
项目协作单位

指导学生已指导学生
邹梓姣硕士研究生085238-生物工程
张楠硕士研究生071005-微生物学
李忆梦博士研究生071005-微生物学
袁传飞博士研究生100102-免疫学
王博博士研究生100103-病原生物学
邵伟博士研究生071005-微生物学
胡亮波博士研究生071005-微生物学
汪习博士研究生071005-微生物学
陈诚硕士研究生071005-微生物学
现指导学生
李玉凤硕士研究生085238-生物工程
马小燕硕士研究生071005-微生物学