基本信息
年锐男博导中国科学院青岛生物能源与过程研究所
电子邮件： nianrui@qibebt.ac.cn
通信地址： 青岛市崂山区松岭路189号
邮政编码： 266101

研究领域高性能蛋白质材料、单克隆抗体蛋白的制备、大规模细胞培养以及蛋白质纯化技术

教育背景
学历08/2003 ~ 08/2008 新加坡国立大学, 新加坡
博士, 化学与分子生物工程系
09/1999 ~ 07/2003 天津大学, 天津, 中国
学士, 化学工程与技术
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工作经历??10/2015 ~ 现在 中科院青岛生物能源与过程研究所，青岛，正高级工程师
06/2012 ~ 09/2015 Bioprocessing Technology Institute, A*STAR, 新加坡, II级研究科学家
08/2010 ~ 05/2012 A-Bio 制药公司，新加坡，研究科学家
01/2010 ~ 07/2010 Temasek Life Sciences Laboratory (TLL)，新加坡，研究科学家
08/2008 ~ 12/2009 德国赛多利斯生物技术有限公司韩国分公司，韩国，高级研究员
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出版信息Fan X, Zhou Y, Yu J, Li F, Chen Q, Song H, Feng D, Liu W, Nian R, Xian M. Non-affinity purification of a nanobody by void-exclusion anion exchange chromatography and multimodal weak cation exchange chromatography. Sep. Purif. Technol, 2019, 225:88-96. (通讯作者)
Zhou D, Sun Y, Bao Z, Liu W, Xian M, Nian R, Xu F. Improved Cell Viability and Biocompatibility of Bacterial Cellulose through in Situ Carboxymethylation. Macromol. Biosci, 2019, **. (通讯作者)
Liu W, Zhou D, Sun Y, Yu J, Chen Q, Bao Z, Fan X, Liang Y, Peng X, Xian M, Nian R. Reduction of chromatin heteroaggregates by acid precipitation of mammalian cell culture and ramification in protein A chromatography for recombinant immunoglobulin G purification. Biochem. Eng. J, 2019, 143, 65-74. (通讯作者)
Liu W, Sun Y, Yu J, Chen Q, Bao Z, Fan X, Liang Y, Peng X, Xian M, Nian R. Advance chromatin extraction improves the performance of electropositive mixed-mode chromatography as a capture step and enables its integration with void-exclusion anion exchange chromatography as a two-column-step purification platform for monoclonal antibody production. Biochem. Eng. J, 2019, 142, 145-152. (通讯作者)
Bao Z, Sun Y, Rai K, Peng X, Wang S, Nian R, Xian M. The promising indicators of the thermal and mechanical properties of collagen from bass and tilapia: synergistic effects of hydroxyproline and cysteine. Biomater. Sci, 2018, 6, 3042-3052. (通讯作者)
Liu W, Song H, Chen Q, Yu J, Xian M, Nian R, Feng D. Recent advances in the selection and identification of antigen-specific nanobodies. Mol. Immunol. 2018, 96, 37-47. (通讯作者)
Chen Q, Toh P, Sun Y, Abdul Latiff SM, Hoi A, Xian M, Zhang H, Nian R, Zhang W, Gagnon P. Histone-dependent IgG conservation in octanoic acid precipitation and its mechanism. Appl. Microbiol. Biotechnol, 2016, 100, 9933-9941. (通讯作者)
Chen Q, Abdul Latiff SM, Toh P, Peng X, Hoi A, Xian M, Zhang H, Nian R, Zhang W, Gagnon P. A simple and efficient purification platform for monoclonal antibody production based on chromatin-directed cell culture clarification integrated with precipitation and void-exclusion anion exchange chromatography. Journal of Biotechnology, 2016, 236, 128-140. (通讯作者)
陈泉，卓燕玲，许爱娜，咸漠，彭馨莹，杨元生，张海波，年锐，张巍.蛋白A亲和层析法纯化单克隆抗体工艺的优化.生物工程学报, 2016, 32, 1-12. (通讯作者)
Nian R, Zhang W, Tan L, Lee J, Bi X, Yang Y, Gan HT, Gagnon P. Advance chromatin extraction improves capture performance of protein A affinity chromatography. J.Chromatogr. A,2016, 1431, 1-7.
Gagnon P, Nian R. Conformational plasticity of IgG during protein A affinity chromatography. J. Chromatogr. A, 2016, 1433, 98-105.
Nian R, Gagnon P. Advance chromatin extraction enhances performance and productivity of cation exchange chromatography-based capture of Immunoglobulin G monoclonal antibodies. J. Chromatogr. A, 2016, 1453, 54-61.
Nian R, Chuah C, Lee J, Gan HT, Latiff S, Lee WY, Vagenende, V, Yang YS, Gagnon P. Void exclusion of antibodies by grafted-ligand porous particle anion exchangers. J. Chromatogr. A. 2013, 1282, 127-132.
Nian R, Kim DS, Nguyen T, Tan L, Kim CW, Yoo IK, Choe WS. Chromatographic biopanning for the selection of peptides with high specificity to Pb2+ from phage displayed peptide library. J. Chromatogr. A. 2010, 1217, 5940-5949.
Nian R, Kim DS, Tan L, Kim CW, Choe WS. Synergistic coordination of polyethylene glycol with ClpB/DnaKJE bichaperone for refolding of heat-denatured malate dehydrogenase. Biotechnol. Prog. 2009, 25, 1078-1085.
Nian R, Tan L, Yoo Ik-Keun, Choe WS. Molecular chaperones enhanced IMAC refolding of gloshedobin, a thrombin-like enzyme from snake venom. J. Chromatogr. A. 2008, 1214, 47-58.
Nian R, Tan L, Choe WS. Folding-like-refolding of heat-denatured MDH using unpurified ClpB and DnaKJE. Biochem. Eng. J. 2008, 40, 35-43.
Nian R, Tan L, Choe WS. Effective reduction of truncated expression of gloshedobin in Escherichia coli using molecular chaperone ClpB. Chem. Eng. Sci. 2008, 63, 2875-2880.
Nian R, Tan L, Choe WS. Polyethyleneimine-mediated chemical extraction of cytoplasmic His-tagged inclusion body proteins from E. coli. Biotechnol. Prog. 2008, 24, 417-425.
Choe WS,Nian R, Lai WB. Recent advances in biomolecular process intensification. Chem. Eng. Sci. 2006, 61, 886-906.
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