基本信息
刘君男博导中国科学院天津工业生物技术研究所
电子邮件： liu_jun@tib.cas.cn
通信地址： 天津空港经济区西七道32号
邮政编码： 300308

研究领域

招生信息
招生专业071005-微生物学

招生方向微生物生理学, 代谢工程


教育背景2002-09--2006-03中国科学院微生物研究所博士学位
1999-09--2002-06南开大学硕士学位
1994-09--1998-06南开大学学士学位

学历
学位

工作经历
工作简历2014-02~现在,中国科学院天津工业生物技术研究所,研究员
2010-07~2013-12,美国西奈山医学院,研究助理教授
2006-03~2010-06,美国西奈山医学院,博士后
2002-09~2006-03,中国科学院微生物研究所,博士学位
1999-09~2002-06,南开大学,硕士学位
1994-09~1998-06,南开大学,学士学位

社会兼职2017-03-09-今,Review editor (Frontier in Extreme Microbiology),
2011-06-01-今,审稿编辑(Reviewing Editor for Frontiers in Antimicrobials, Resistance and Chemotherapy),


教授课程

专利与奖励
奖励信息
专利成果（ 1 ）  一种编码钠氢泵蛋白基因的核苷酸序列及其应用., 2010, 第 2 作者, 专利号: CN6.9
（ 2 ） 一种新型谷氨酸脱羧酶基因的克隆及其应用, 2015, 第 1 作者, 专利号: 1.0


出版信息
发表论文（1）Metabolic engineering of Corynebacterium glutamicum for L-cysteine production.,Appl Microbiol Biotechnol.,2019,第11作者
（2）Impaired oxidative stress and sulfur assimilation contribute to acid tolerance of Corynebacterium glutamicum.,Appl Microbiol Biotechnol.,2019,第11作者
（3）Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate from glucose and CO2.,Microb Cell Fact.,2019,第3作者
（4）Recent advances in the applications of promoter engineering for the optimization of metabolite biosynthesis.,World J Microbiol Biotechnol,2019,第11作者
（5）Combining Protein and Metabolic Engineering Strategies for High-Level Production of O-Acetylhomoserine in Escherichia coli.,ACS Synth Biol.,2019,第11作者
（6）Promoter library-based module combination (PLMC) technology for optimization of threonine biosynthesis in Corynebacterium glutamicum.,Appl Microbiol Biotechnol.,2018,第11作者
（7）The Lysine 299 Residue Endows the Multisubunit Mrp1 Antiporter with Dominant Roles in Na+ Resistance and pH Homeostasis in Corynebacterium glutamicum.,Appl Environ Microbiol,2018,第11作者
（8）Roles of Staphylococcus aureus Mnh1 and Mnh2 Antiporters in Salt Tolerance, Alkali Tolerance, and Pathogenesis,J Bacteriol,2018,第4作者
（9）Biotechnological advances and perspectives of gamma-aminobutyric acid production.,World J Microbiol Biotechnol.,2017,第11作者
（10）Co-expression of L-glutamate oxidase and catalase in Escherichia coli to produce α-ketoglutaric acid by whole-cell biocatalyst.,Biotechnol Lett.,2017,第11作者
（11）外源精氨酸对谷氨酸棒杆菌在高葡萄糖胁迫下生长和发酵特性的影响,微生物学通报,2017,第11作者
（12）Expression, characterization and mutagenesis of a novel glutamate decarboxylase from Bacillus megaterium,Biotechnol Lett,2016,第11作者
（13）In vitro functional characterization of the Na+/H+ antiporters in Corynebacterium glutamicum.,FEMS Microbiol Lett,2016,第11作者
（14）细菌 Na+/H+ 逆向转运蛋白的研究进展,微生物学通报,2015,第11作者
（15）The c-ring ion binding site of the ATP synthase from Bacillus pseudofirmus OF4 is adapted to alkaliphilic lifestyle,Mol Microbiol,2014,第4作者
（16）Cardiolipin Is Dispensable for Oxidative Phosphorylation and Non-fermentative Growth of Alkaliphilic Bacillus pseudofirmus OF4 ,J. Biol. Chem,2014,第1作者
（17）The c-ring stoichiometry of ATP synthase is adapted to cell physiological requirements of alkaliphilic Bacillus pseudofirmus OF4,Proc Natl Acad Sci USA,2013,第4作者
（18）Roles of AtpI and two YidC-type proteins from alkaliphilic Bacillus pseudofirmus OF4 in ATP synthase assembly and nonfermentative growth,J Bacteriol,2013,第1作者
（19）Genome of alkaliphilic Bacillus pseudofirmus OF4 reveals adaptations that support the ability to grow in an external pH range from 7.5 to 11.4,Environ Microbiol,2011,第4作者
（20）Mutations in a helix-1 motif of the ATP synthase c-subunit of Bacillus pseudofirmus OF4 cause functional deficits and changes in the c-ring stability and mobility on sodium dodecyl sulfate-polyacrylamide gel electrophoresis,Biochemistry,2011,第1作者
（21）The ATP synthase a-subunit of extreme alkaliphiles is a distinct variant: mutations in the critical alkaliphile-specific residue Lys-180 and other residues that support alkaliphile oxidative phosphorylation,J Biol Chem,2010,第3作者
（22）F1F0-ATP synthases of alkaliphilic bacteria: lessons from their adaptations.,Biochim Biophys Acta,2010,第2作者
（23）Characterization of the Functionally Critical AXAXAXA and PXXEXXP Motifs of the ATP Synthase c-Subunit from an Alkaliphilic Bacillus,J Biol Chem,2009,第1作者
（24）Purification of two putative type II NADH dehydrogenases with different substrate specificities from alkaliphilic Bacillus pseudofirmus OF4,Biochim Biophys Acta,2008,第1作者
（25）Three putative cation/proton antiporters from the soda lake alkaliphile Alkalimonas amylolytica N10 complement an alkali-sensitive Escherichia coli mutant,Microbiology,2007,第2作者
（26）The activity profile of the NhaD-type Na+(Li+)/H+ antiporter from the soda Lake Haloalkaliphile Alkalimonas amylolytica is adaptive for the extreme environment,J Bacteriol,2005,第1作者

发表著作（ 1 ） 极端嗜碱菌的适应机制(极端微生物书章节),Adaptive mechanisms of extreme alkaliphiles. In: Extremophiles Handbook,Springer,2010-02,第2作者


科研活动
科研项目（ 1 ） 微生物耐受胁迫环境的生理机制, 主持,部委级,2015-10--2018-09
（ 2 ） 二氧化碳的人工生物转化（人工电子载体的设计与优化）, 参与,部委级,2016-03--2019-03
（ 3 ） 谷氨酸棒杆菌ATP合成酶c环的组装及其功能研究, 主持,省级,2017-04--2020-03
（ 4 ） 电能细胞设计与构建, 参与,国家级,2019-07--2024-06
（ 5 ） 生物酶法催化合成7-ADCA, 参与,院级,2019-07--2022-06

参与会议（1）谷氨酸棒杆菌抵御环境胁迫的生理机制解析中国微生物学会学术年会2017-10-20
（2）Cardiolipin is not essential for respiration-dependent ATP synthesis that supports malate growth of an alkaliphilic Bacillus at pH 7.5 to 10.5 113届微生物学年会(美国)Liu J, Ryabichko S, Fackelmayer OJ, Bogdanov M, Dowhan W, Krulwich TA2013-05-18
（3）YidC-homologues in Bacillus pseudofirmus OF4 show different pH profiles for their rolesLiu J2012-02-19
（4）Effects of deletions in the atp locus and yidC-like genes on the ATP synthase in an alkaliphilic BacillusLiu J2011-07-17


合作情况
项目协作单位

指导学生