基本信息
毕昌昊 中国科学院天津工业生物技术研究所
电子邮件：bi_ch@tib.cas.cn
通信地址：天津市空港经济区西七道32号
邮政编码：300308


研究领域
毕昌昊 中国科学院天津工业生物技术研究所研究员，微生物合成生物技术方法研究组PI，“国家863项目”首席科学家。
本科及硕士毕业于南开大学，2009年于弗罗里达大学取得博士学位，先后在特拉华大学和劳伦斯伯克利国家实验室工作，2014年开始在中科院天津工生所工作。主要研究方向为合成生物学。现从事合成生物学技术方法的创建与运用，基因组编辑技术研发，新型底盘细胞设计构建等方面的研究。几种代表性的合成生物学和基因组编辑技术方法造成较大影响，上百个国内外实验室已索取材料并使用。在主流学术期刊Metab Eng, ACS Synth., Bio.l, Microb. Cell Fact., ChemComm., J. Bacteriol., 和AEM等发表SCI论文32篇，申请国际专利一项。在应用研究方面，通过和所内研究组的合作攻关，目前番茄红素、β-胡萝卜素及其它几个类胡萝卜素细胞工厂的发酵生产性能都达到国际先进水平。番茄红素、β-胡萝卜和虾青素的大肠杆菌细胞工厂合成技术已转化企业进行商业生产，将对萜类化合物生产技术提升产生重大影响。


招生信息招收分子生物学，微生物学，遗传学等研究和学习背景学生。欢迎联合培养，客座硕士研究生或博士研究生到研究组工作学习，研究组项目丰富，支持充足，并对所有学生有相对优厚补助。

招生专业071005-微生物学
071010-生物化学与分子生物学
071020-生物技术与医药

招生方向 微生物生理学
合成生物学
代谢工程

教育背景2004-08--美国弗罗里达大学博士
2001-09--南开大学硕士
1997-09--南开大学学士

学历
学位

工作经历
工作简历2014-01~现在,中国科学院天津工业生物技术研究所,研究员、教授
2011-09~现在,美国劳伦斯伯克利国家实验室,青年研究员
2009-09~现在,美国特拉华大学,博士后研究员

社会兼职

教授课程 代谢工程导论

专利与奖励
奖励信息 （1） 总统奖学金，一等奖，研究所（学校）级，2005

专利成果

出版信息Wang, W., He, P., Zhao, D., Ye, L., Dai, L., Zhang, X*., Bi, C*. (2019). Construction of Escherichia coli cell factories for crocin biosynthesis. Microbial cell factories, 18(1), 120. 二区
Wu, T., Li, S, Zhang, X*,Bi, C*. (2019). Engineering an Artificial Membrane Vesicle Trafficking System (AMVTS) for the Excretion of β-Carotene in Escherichia coli. ACS Synth. Biol 二区
Wu, Z., Wang, J., Zhang, Zhang, X*,Bi, C*. (2019). Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate by increasing the intracellular FAD pool. Biochemical Engineering Journal. 146(132-142) 二区
Wu, Z., Wang, J., Liu, J., Wang, Y., Bi, C*., Zhang, X*. (2019). Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate from glucose and CO 2.Microbial cell factories,18(1), 15. 二区
Li, Z., Xiong, B., Liu, L., Li, S., Xin, X., Li, Z., Zhang, X*, Bi, C*. (2019). Development of an autotrophic fermentation technique for the production of fatty acids using an engineered Ralstonia eutropha cell factory.Journal of industrial microbiology & biotechnology, 1-8. 二区
Ye, L., Zhu, X., Wu, T., Wang, W., Zhao, D., Bi, C. *., Zhang, X*. (2018). Optimizing the localization of astaxanthin enzymes for improved productivity. Biotechnology for biofuels, 11(1), 278. 一区
Xiong, B., Li, Z., Liu, L., Zhao, D., Zhang, X*., Bi, C*. (2018). Genome editing of Ralstonia eutropha using an electroporation-based CRISPR-Cas9 technique.Biotechnology for Biofuels,11(1), 172. 一区
Wu, T., Li, S., Zhang, B., Bi C*, Zhang X*. (2018). Engineering Saccharomyces cerevisiae for the production of the valuable monoterpene ester geranyl acetate.Microbial cell factories,17(1), 85. 二区
Wu, T., Ye, L., Zhao, D., Li, S., Li, Q., Zhang, B*., Bi, C*. (2018). Engineering membrane morphology and manipulating synthesis for increased lycopene accumulation in Escherichia coli cell factories.3 Biotech,8(6), 269. 三区
Feng, X., Zhao, D., Zhang, X., Ding, X*., & Bi, C*. (2018). CRISPR/Cas9 assisted Multiplex Genome Editing Technique in Escherichia coli.Biotechnology journal, **. 二区
Zhao, D., Feng, X., Zhu, X., Wu, T., Zhang, X*., and Bi, C*. (2017) CRISPR/Cas9-assisted gRNA-free one-step genome editing with no sequence limitations and improved targeting efficiency, Scientific Reports. 2017. 16624. 三区

Zhu X, Zhao D, Qiu H, Fan F, Man S, Bi C*, Zhang X*(2017). The CRISPR/Cas9-facilitated multiplex pathway optimization (CFPO) technique and its application to improve the Escherichia coli xylose utilization pathway. Metab Eng. 43:37-45. 一区
Wu T, Ye L, Zhao D, Li S, Li Q, Bi C*, Zhang X*. (2017) Membrane engineering - A novel strategy to enhance the production and accumulation of β-carotene in Escherichia coli. Metab Eng., 43: 85-91. 一区
Li Q, Fan F, Gao X, Yang C, Bi C, Tang J, Liu T, Zhang X*. (2017) Balanced activation of IspG and IspH to eliminate MEP intermediate accumulation and improve isoprenoids production in Escherichia coli. Metab Eng.08.005 一区
Ye L, He P, Li Q, Zhang X*, Bi C*. (2017) Type IIs restriction based combinatory modulation technique for metabolic pathway optimization. Microb Cell Fact. 16:47. 二区
Xiao M, Zhu X, Xu H, Tang J, Liu R, Bi C, Fan F*, Zhang X*. (2017) A novel point mutation in RpoB improves osmotolerance and succinic acid production in Escherichia coli. BMC Biotechnology. 17:10 二区
Li J, Zhu, X Chen, J Zhao, D., Zhang X*, Bi, C*. (2017) Construction of a novel anaerobic pathway in Escherichia coli for propionate production. BMC biotechnology17(1), 38. 二区
Zhao D, Yuan S, Xiong B, Sun H, Ye L, Li J, Zhang X*, Bi C*. (2016) Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9 Microb Cell Fact. 15:205. 二区
Ye L, Zhang C, Bi C, Li Q*, Zhang X*. (2016) Combinatory Optimization of Chromosomal Integrated Mevalonate Pathway for β-carotene Production in Escherichia coli. Microb Cell Fact. 2016, 15:202.6 二区
Li S, Ding W, Zhang X, Jiang H*, Bi C*. (2016) Development of a modularized two-step (M2S) chromosome integration technique for integration of multiple transcription units in Saccharomyces cerevisiae. Biotechnol Biofuels. 2016, 9:232 一区
Sun H, Zhao D, Xiong B, Zhang C*, Bi C*. (2016). Engineering Corynebacterium glutamicum for violacein hyper production. Microb Cell Fact.15(1), 148. 二区
C. Bi, J. Mueller, Y. Yeh, N. J. Hillson, H. R. Beller, S. R. Chhabra, and S. W. Singer. (2013) Developing and Applying a Broad-Host Synthetic Biology Tool Box for Metabolic Engineering of Ralstonia eutropha for Hydrocarbon Production. Microb Cell Fact, 12:107 二区
C. Bi, S. W. Jones, Dan R. Hess, B. P. Tracy, and E. T. Papoutsakis. (2011) SpoIIE is necessary for asymmetric division, sporulation, and the expression of σF, σE, and σG but does not control solvent production in Clostridium acetobutylicum. J Bacter. 193 no. 19 5130-5137 二区

C. Bi, J. D Rice, J. F Preston. (2009) Complete fermentation of xylose and methylglucuronoxylose derived from methylglucuronoxylan by Enterobacter asburiae strain JDR-1. Applied and Environmental Microbilogy. (1),395-404 二区

C. Bi, X. Zhang, L. O. Ingram, J. F Preston. (2009) Genetic engineering Enterobacter asburiae strain JDR-1 for efficient ethanol production from hemicellulose hydrolysate. Applied and Environmental Microbilogy. (9),5743-5749 二区

C. Bi, X. Zhang, L. O. Ingram, J. F Preston. (2009) Genetic engineering of Enterobacter asburiae strain JDR-1 for efficient latic acid production from hemicellulose hydrolysate. Biotechnology Letters. (31) 1551-1557 三区
Y Yuan ， C. Bi ， SA. Nicolaou ，K A. Zingaro ，M Ralston ，ET. Papoutsakis Overexpression of the Lactobacillus plantarum peptidoglycan biosynthesis murA2 gene increases the tolerance of Escherichia coli to alcohols and enhances ethanol production. Applied Microbiology and Biotechnology. 2014. 98:8399–8411. 二区
G. Linshiz, N. Stawski, S. Poust, C. Bi, J. D. Keasling, and N. J. Hillson. PaR-PaR：Cross-Platform Laboratory Automation system. ACS Synth. Biol, 2014,3,515-524 二区
Y. Yeh, J. Muller, C. Bi, N. J. Hillson, H. R. Beller, S. R. Chhabra, and S. W. Singer. Functionalizing Bacterial Cell Surfaces with a Phage Protein. ChemComm, 2013,49, 910-912一区
G. Linshiz, N. Stawski, S. Poust, C. Bi, J. D. Keasling, and N. J. Hillson. PaR-PaR Laboratory Automation platform. ACS Synth. Biol, 2012,2,216-222 二区
G. Linshiz, E. Jensen,N. Stawski, C. Bi, J. D. Keasling, and N. J. Hillson.End-to-end automated microfluidic platform for synthetic biology: from design to functional analysis. Journal of Biological Engineering. 2016. 10:3 二区



发表著作

科研活动
科研项目 （1） 先进生物制造工程菌株的构建与应用，参与，院级级，2014-01--2015-12

参与会议

合作情况
项目协作单位诺和诺德中国研究中心等


指导学生现指导学生
李智**
熊斌**
叶立军**