基本信息
汪海林男博导中国科学院生态环境研究中心
电子邮件： HLWang@rcees.ac.cn
通信地址： 北京市海淀区双清路18号
邮政编码： 100085

研究领域

招生信息
招生专业070302-分析化学
0710Z1-基因组学

招生方向高灵敏生物分析
DNA损伤与修复
表观遗传学


教育背景1994-09--1997-06中科院大连化学物理研究所理学博士
1991-09--1994-08中科院大连化学物理研究所理学硕士
1987-09--1991-07武汉大学化学系理学学士

学历
学位

工作经历
工作简历2005-12~现在,中科院生态环境研究中心,研究员、博士生导师
2000-07~2005-12,University of Alberta,postdoctoral fellow, research associate
1997-07~2000-07,中科院大连化物所,助研、副研

社会兼职2018-01-01-2020-12-31,Analytical Chemistry, 编委
2017-10-01-今,Journal of Environmental Sciences, 编委
2016-03-01-今,北京理化分析测试协会色谱分会, 副理事长
2016-01-01-今,Genomics, Proteomics & Bioinformatics, 编委
2013-08-01-今,J Sep Sci, 编委
2013-01-01-今,《中国科学?化学》, 编委
2010-01-01-今,《环境化学》, 编委


教授课程前沿讲座
环境与健康
环境健康前沿研究
环境与健康前沿研究


专利与奖励
奖励信息（1） 中国分析测试协会科学技术奖, 特等奖, 其他, 2015
（2） 中科院优秀研究生导师奖, 院级, 2015
（3） 中国分析测试协会科学技术奖, 一等奖, 其他, 2013
（4） 中科院优秀研究生指导教师奖, 院级, 2013
（5） 中科院优秀研究生导师奖, 院级, 2012
（6） 中国分析测试协会科学技术奖, 一等奖, 其他, 2010
（7） 中国教育部自然科学奖, 一等奖, 部委级, 2007
（8） 中科院院长奖学金特别奖, 特等奖, 其他, 1997

专利成果（ 1 ） 脂蛋白毛细管涂层及其制备方法, 发明, 2008, 第 1 作者, 专利号: 5.9
（ 2 ） 荧光偏振增强免疫毛细管电泳分析, 发明, 2009, 第 1 作者, 专利号: 6.7
（ 3 ） 量子点增强的高灵敏DNA加合物分析方法, 发明, 2009, 第 1 作者, 专利号: .2
（ 4 ） 毛细管电泳-柱上激光诱导荧光偏振检测装置, 发明, 2009, 第 1 作者, 专利号: 2.9
（ 5 ） 一种用于DNA酶切为单核苷的级联酶反应器, 发明, 2016, 第 1 作者, 专利号: 4.7


出版信息
发表论文（1）An alternative culture method to maintain genomic hypomethylation of mouse embryonic stem cells using MEK inhibitor PD** and vitamin C,J Vis Exp,2018,通讯作者
（2）METTL3-mediated m6A modification is required for cerebellar development.,PLoS Biol.,2018,其他（合作组作者）
（3）Global DNA 5-hydroxymethylcysotine and 5-formylcytosine contents are decreased in the early stage of hepatocellular carcinoma.,Hepatology,2018,通讯作者
（4）Multienzyme cascade bioreactor for a 10 min digestion of genomic DNA into single nucleosides and quantitative detection of structural DNA modifications in cellular genomic DNA,ACS Appl Mater Interfaces,2018,通讯作者
（5）Vertical ultrafiltration-facilitated DNA digestion for rapid and sensitive UHPLC-MS/MS detection of DNA modifications,Analytical Chemistry,2018,通讯作者
（6）Predominance of N6-methyladenine-specific DNA fragments enriched by multiple immunoprecipitation,Analytical Chemistry,2018,通讯作者
（7）Affinity maturation of an antibody for the UV-induced DNA lesions 6,4 pyrimidine-pyrimidones,Appl Microbiol Biotechnol.,2018,通讯作者
（8）Nickel(ii) inhibits the oxidation of DNA 5-methylcytosine in mammalian somatic cells and embryonic stem cells,Metallomics,2018,通讯作者
（9）Roles of the CSE1L-mediated nuclear import pathway in epigenetic silencing,Proc Natl Acad Sci USA,2018,通讯作者
（10）Small molecules capable of activating DNA methylation-repressed genes targeted by the p38 mitogen-activated protein kinase pathway,J Biol Chem,2018,其他（合作组作者）
（11）MALDI-MS Imaging Reveals Asymmetric Spatial Distribution of Lipid Metabolites from Bisphenol S-Induced Nephrotoxicity,Analytical Chemistry,2018,第4作者
（12）Reduced self-diploidization and improved survival of semi-cloned mice produced from androgenetic haploid embryonic stem cells through overexpression of Dnmt3b.,Stem Cell Reports,2018,其他（合作组作者）
（13）UHPLC-Q-TOF/MS detection of UV-induced TpT dimeric lesions in genomic DNA,J Chromatogr. B,2018,通讯作者
（14）Elevated 8-oxo-7,8-dihydro-2'-deoxyguanosine in genome of T24 bladder cancer cells induced by halobenzoquinones,J Environ Sci (China),2018,通讯作者
（15）ATpase activity tightly regulates RecA nucleofilaments to promote homologous recombination.,Cell Discovery,2017,通讯作者
（16）5-methylcytosine promotes mRNA export – NSUN2 as the methyltransferases and ALYREF as an m5C reader,Cell Research,2017,通讯作者
（17）Nickel (II) inhibits Tet-mediated 5-methylcytosine oxidation by high affinity displacement of the cofactor iron (II),ACS Chemical Biology,2017,通讯作者
（18）Metabolically generated stable isotope-labeled deoxynucleoside code for tracing DNA N6-methyladenine in human Cells.,Analytical Chemistry,2017,通讯作者
（19）Immunofluorescence imaging strategy for evaluation of the accessibility of DNA 5-hydroxymethylcytosine in chromatins.,Analytical Chemistry,2017,通讯作者
（20）Detection of 8-hydroxydeoxyguanosine (8-OHdG) as a biomarker of oxidative damage in peripheral leukocyte DNA by UHPLC–MS/MS,Journal of Chromatography B,2017,通讯作者
（21）Three-Enzyme Cascade Bioreactor for Rapid Digestion of Genomic DNA into Single Nucleosides.,Anal Chem,2016,通讯作者
（22）MEK inhibitor PD** and vitamin C synergistically induce hypomethylation of mouse embryonic stem sells.,Oncotarget,2016,通讯作者
（23）N6-methyladenine DNA modification in Drosophila,Cell,2015,通讯作者
（24）Nanoparticles-free fluorescence anisotropy amplification assay for detection of RNA nucleotide-cleaving DNAzyme activity.,Anal Chem,2015,通讯作者
（25）Detection of human urinary 5-hydroxymethylcytosine by stable isotope dilution HPLC-MS/MS analysis.,Anal Chem,2015,通讯作者
（26）Transient isotachophoresis focusing of DNA and DNA-protein complexes is essentially enhanced by spontaneously dissolved aerial carbon dioxide in electrolytes,Anal Chem,2015,通讯作者
（27）Boronic acid-mediated polymerase chain reaction for gene- and fragment-specific detection of 5-hydroxymethylcytosine,Nucleic Acids Research,2014,通讯作者
（28）Redox-active quinones induces genome-wide DNA methylation changes by an iron-mediated and Tet-dependent mechanism,Nucleic Acids Research,2014,通讯作者
（29）Interplay of binding stoichiometry and recognition specificity for the interaction of MBD2b protein and methylated DNA revealed by affinity capillary electrophoresis coupled with laser-induced fluorescence analysis.,Anal Chem,2014,通讯作者
（30）Ascorbic Acid Enhances Tet-mediated 5-Methylcytosine Oxidation and Promotes DNA Demethylation in Mammals.,J. Am. Chem. Soc,2013,通讯作者
（31）Imaging of Non-uniform Motion of Single DNA Molecules Reveals the Kinetics of Varying-Field Isotachophoresis.,J. Am. Chem. Soc.,2013,通讯作者
（32）An ammonium bicarbonate-enhanced stable isotope dilution UHPLC-MS/MS method for sensitive and accurate quantification of acrolein-DNA adducts in human leukocytes.,Anal Chem,2013,通讯作者
（33）Potent DNA damage by polyhalogenated quinones and H?O? via a metal-independent and Intercalation-enhanced oxidation mechanism.,Scientific Reports,2013,通讯作者
（34）Polyvinyl pyrrolidone promotes DNA cleavage by a ROS-independent and depurination mechanism.,Environ Sci Technol,2013,通讯作者
（35）Size-dependent toxicity of nano-C60 aggregates: More sensitive indication by apoptosis-related Bax translocation in cultured human cells.,Environm. Sci. & Technol.,2012,通讯作者
（36）Specific and sensitive fluorescence anisotropy sensing of guanine-quadruplex structures via a photoinduced electron transfer mechanism.,Anal Chem,2012,通讯作者
（37）Fluorescence anisotropy reduction of allosteric aptamer for sensitive and specific protein signaling.,Anal Chem,2012,通讯作者
（38）Capillary monolithic bioreactor of immobilized snake venom phosphodiesterase for mass spectrometry based oligodeoxynucleotide sequencing.,Anal Chem,2012,通讯作者
（39）Fluorescence anisotropy analysis for mapping aptamer-protein interaction at the single nucleotide level,J. Am. Chem. Soc.,2011,通讯作者
（40）Enhanced bacterial biosensor for fast and sensitive detection of oxidatively DNA damaging agents,Anal. Chem.,2011,通讯作者
（41）Inhibition of polymerase activity by pristine fullerene nanoparticles can be mitigated by abundant proteins,Chem. Comm.,2010,通讯作者
（42）Metal cation mediated capillary electrophoresis of nucleic acids,Anal. Chem.,2010,通讯作者
（43）Fluorescently imaged particle counting immunoassay for sensitive detection of DNA modifications,Anal. Chem.,2010,通讯作者
（44）Fluorescently imaged particle counting immunoassay for sensitive detection of DNA modifications.,Anal. Chem.,2010,通讯作者
（45）Synthesis and characterization of DNA fluorescent probes containing a single site-specific stereoisomer of anti-benzo[a]pyrene diol epoxide-N2-dG,Chemical Research in Toxicology,2009,通讯作者
（46）DNA wrapping is required for DNA damage recognition in the Escherichia coli DNA nucleotide excision repair pathway.,Proc. Natl. Acad. Sci. USA,2009,第1作者
（47）Fluorescence polarization combined capillary electrophoresis immunoassay for rapid and sensitive detection of genomic DNA methylation,Anal. Chem.,2009,通讯作者
（48）Quantum dots enhanced ultrasensitive detection of DNA adducts,Anal. Chem.,2009,通讯作者
（49）Organic osmolyte mediated kinetic capillary electrophoresis for study of protein-DNA interactions,Anal. Chem.,2009,通讯作者
（50）A microdialysis sampling method for evaluation of binding kinetics of small molecules to macromolecules,Anal. Chem.,2008,通讯作者

发表著作（1）现代毒理学,A Textbook of Mordern Toxicology,中国科学出版社,2011-06,第2作者


科研活动
科研项目（ 1 ） 国家基金, 主持,国家级,2012-01--2015-12
（ 2 ） 毛细管电泳-双光子荧光偏振单分子检测装置, 主持,国家级,2014-01--2017-12
（ 3 ） DNA损伤分析方法与生物效应研究及专用仪器开发”, 主持,国家级,2012-01--2015-12
（ 4 ） 针对蛋白质-核酸相互作用研究的单分子荧光偏振分析方法学与技术, 主持,国家级,2015-01--2019-12
（ 5 ） 污染物与生物大分子的交互作用, 主持,部委级,2014-06--2018-12

参与会议

合作情况
项目协作单位

指导学生已指导学生
刘淑芳博士研究生083001-环境科学
王智鑫博士研究生083001-环境科学
冯峰博士研究生083001-环境科学
李涛博士研究生083001-环境科学
王超硕士研究生083001-环境科学
陈芝兰博士研究生083001-环境科学
宋玉玲硕士研究生083001-环境科学
王晓利博士研究生083001-环境科学
章大鹏博士研究生083001-环境科学
刘胜权博士研究生083001-环境科学
赵超博士研究生083001-环境科学
尹瑞川博士研究生083001-环境科学
李文静硕士研究生083001-环境科学
邹丹丹博士研究生083001-环境科学
李翠平博士研究生083001-环境科学
赵柏林博士研究生070302-分析化学
黄华博士研究生083001-环境科学
刘保东博士研究生083001-环境科学
刘晓玲博士研究生070302-分析化学
刘迪硕士研究生077601-环境科学
吴丹妮博士研究生083001-环境科学
赖玮毅博士研究生083001-环境科学
朱会宇博士研究生083001-环境科学
现指导学生
袁征博士研究生070302-分析化学
陈政博士研究生083001-环境科学
任云博士研究生083001-环境科学
莫杰珍博士研究生070302-分析化学
李瑶硕士研究生071010-生物化学与分子生物学
刘艳博士研究生071007-遗传学
景昶衡硕士研究生077601-环境科学
余方志博士研究生070302-分析化学
孙境蔚硕士研究生071010-生物化学与分子生物学
郑婧硕士研究生070302-分析化学
吕聪博士研究生070302-分析化学