基本信息
郭惠珊 女 汉族 博导 微生物研究所
电子邮件： guohs@im.ac.cn
联系电话： 010-6484 7989
手机号码：
通信地址：北京市朝阳区北辰西路1号院
邮政编码：100101


研究领域RNA沉默是一种在动植物中广泛存在的抵抗外源RNA和病毒的适应性免疫反应机制。能产生信号并系统运输是植物RNA沉默最重要的特征，即系统RNA沉默，也是植物抗病毒最关键的一环。另外，siRNA参与DNA甲基化和染色质重塑，使RNA沉默成为表观遗传学 (Epigenetic)研究领域的重要课题。本研究组以模式植物拟南芥、烟草和重要的经济作物棉花为寄主材料，以病毒和真菌为病原微生物，围绕植物RNA沉默和miRNA调控途径，以及其他受病原影响的调控途径开展病原微生物－寄主的相互作用研究，剖析植物致病机理。目前开展以下几方面的研究:
1. 植物抗病RNA沉默机制；
2. 病毒沉默抑制子和病毒卫星RNA的致病性研究；
3. 小RNA介导的RNA沉默的抗病应用研究。
招生信息

招生专业 遗传学



招生方向 植物RNA沉默及其抗病应用


教育背景

学历中山大学生物学，学士


学位西班牙马德里大学， 博士


出国学习工作1992-1993 西班牙马德里大学"Severo Ochoa"分子生物学中心，Guest Investigator
1997-1998 西班牙马德里大学国家生物工程中心（CSIC）博士后

工作经历

工作简历1998-2003 新加坡国立大学分子农业生物学院/淡马锡生命科学研究院Research Fellow
2003-2004 新加坡国立大学淡马锡生命科学研究院，Acting PI
2004-至今 中国科学院"百人计划"，中国科学院微生物所 研究员、博士生导师

社会兼职中国植物病理学会第九届理事会理事
教授课程
专利与奖励

奖励信息2005年国家杰出青年科学基金获得者


专利成果郭惠珊, 段成国, 王春晗
一种培育抗黄瓜花叶病毒植物的方法
申请号：ZL 2007 1 **.3
申请日期：2007年05月25日
公开号：**A
授权公告日：2010年07月21日

郭惠珊, 段成国
RNA诱导的沉默复合体介导的剪切位点及其应用
申请号：ZL 2008 1 **.X
申请日期：2008年07月29日
公开号：**A
授权公告日：2010年12月15日

郭惠珊，周邦军
来自大丽轮枝菌的致病相关蛋白VdGARP1及其编码基因
申请号：**75
申请日期：2010年4月21日
出版信息

发表论文34. Weina Hou#, Chengguo Duan#, Rongxiang Fang, Xiaoyang Zhou*, Huishan Guo*. Satellite RNA reduces expression of the 2b suppressor protein resulting in the attenuation of symptoms caused by Cucumber mosaic virus infection. Molecular Plant Pathology. (2011). (# These authors contribute equally to this work.)

33. Zhonghui Zhang, Hao Chen, Xiahe Huang, Ran Xia, Qingzhen Zhao, Jianbin Lai, Kunling Teng, Yin Li, Liming Liang, Quansheng Du, Xueping Zhou, Huishan Guo, and Qi Xie. BSCTV C2 Attenuates the Degradation of SAMDC1 to Suppress DNA Methylation-Mediated Gene Silencing in Arabidopsis. The Plant Cell.23(1):273-88. (2011).

32. Feng Gao1,2., Bang-Jun Zhou1,3., Guo-Ying Li2, Pei-Song Jia2, Hui Li2, Yun-Long Zhao1,3, Pan Zhao1,3,Gui-Xian Xia1, Hui-Shan Guo1*. A Glutamic Acid-Rich Protein Identified in Verticillium dahliae from an Insertional Mutagenesis Affects Microsclerotial Formation and Pathogenicity. PLOS ONE.5(12):e15319. (2010).

31. Xiaobao Ying, Li Dong, Hui Zhu, Chengguo Duan, Quansheng Du, Dianqiu Lv, Yuanyuan Fang, Juan Antonio Garcia, Rongxiang Fang and Huishan Guo*. RNA-dependent RNA polymerase 1 from Nicotiana tabacum suppresses RNA silencing and enhances viral infection in N. benthamiana. The Plant Cell. 22(4):1358-72. (2010).

30. Lijing Liu, Yiyue Zhang, Sanyuan Tang, Qingzhen Zhao, Zhonghui Zhang, Huawei Zhang, Li Dong, Huishan Guo and Qi Xie*. An efficient system to detect protein ubiquitination by argoinfiltration in Nicotiana benthamiana. The Plant Journal 61:893-903. (2010).

29. Hao Chen, Zhonghui Zhang, Kunling Teng, Jianbin Lai, Yiyue Zhang, Yiliang Huang, Yin Li, Liming Liang, Yiqin Wang, Chengcai Chu, Huishan Guo and Qi Xie*. Up-regulation of LSB1/GDU3 impacts geminivirus infection by activating the salicylic acid pathway. The Plant Journal 62:12-23. (2010).

28. Kunling Teng, Hao Chen, Jianbin Lai, Zhonghui Zhang, Yuanyuan Fang, Ran Xia, Xueping Zhou, Huishan Guo, Qi Xie*. Involvement of C4 Protein of Beet Severe Curly Top Virus (Family Geminiviridae) in Virus Movement. PLoS ONE 5(6), e11280. (2010).

27. Weiquan Wang, Yaorong Wu, Yin Li, Jiaying Xie, Zhonghui Zhang, Zhiyong Deng, Yiyue Zhang, Cuiping Yang, Jianbin Lai, Huawei Zhang, Hongyan Bao, Sanyuan Tang,Chengwei Yang, Peng Gao, Guixian Xia, Huishan Guo and Qi Xie*.A large insert Thellungiella halophila BIBAC library for genomics and identification of stress tolerance genes. Plant Molecular Biology 72:91-99. (2010).

26. Yan Li , Xiuying Liu , Li Huang , Huishan Guo*, Xiu-Jie Wang*. Potential coexistence of both bacterial and eukaryotic small RNA biogenesis and functional related protein homologs in Archaea. Journal of Genetics and Genomics 37: 493-503. (2010).

25. Jianbin Lai, Hao Chen, Kunling Teng, Qingzhen Zhao, Zhonghui Zhan, Yin Li, Liming Liang, Ran Xia, Yaorong Wu, Huishan Guo and Qi Xie. RKP, a RING finger E3 ligase induced by BSCTV C4 protein, affects geminivirus infection by regulation of the plant cell cycle. The Plant Journal 57(5): 905-917. (2009).

24. Zheng, Nuoyan, Xia, Ran, Yang, Cuiping, Yin, Bojiao, Li, Yin, Duan, Chengguo, Liang, Liming, Guo, Huishan, Xie, Qi. Boosted expression of the SARS-CoV nucleocapsid protein in tobacco and its immunogenicity in mice. Vaccine, 27(36): 5001-5007. (2009).

23. Cheng-Guo Duan, Chun-Han Wang, Rong-Xiang Fang and Hui-Shan Guo. Artificial microRNAs highly accessible to targets confer efficient virus resistance in plants. Journal of Virology 82:11084-11095. (2008).

22. Xiang-Feng He, Yuan-Yuan Fang, Lei Feng and Hui-Shan Guo. Characterization of conserved and novel microRNAs and their targets, including a TuMV-induced TIR-NBS-LRR class R gene-derived novel miRNA in Brassica. FEBS letter 582:2445-2452. (2008).

21. Cheng-Guo Duan, Chun-Han Wang and Hui-Shan Guo. Delayed resistance to Cucumber Mosaic Virus mediated by 3’UTR derived hairpin RNA. Chinese Science Bulletin 53:3301-3310. (2008).

20. Jianbin Lai, Hao Chen, Kunling Teng, Qingzhen Zhao, Zhonghui Zhan, Yin Li, Liming Liang, Ran Xia, Yaorong Wu, Huishan Guo and Qi Xie. RKP, a RING finger E3 ligase induced by BSCTV C4 protein, affects geminivirus infection by regulation of the plant cell cycle. The Plant Journal 57(5):905-17. (2008).

19. Zhong-Hui Zhang, Qi Xie and Hui-Shan Guo. Antiviral Defense in Plants. Plant Viruses　 Global Science Books. 1: 21-26 Invited mini-review (2007)

18. Quan-Sheng Du, Cheng-Guo Duan, Zhong-Hui Zhang, Yuan-Yuan Fang, Rong-Xiang Fang, Qi Xie, and Hui-Shan Guo. DCL4 targets Cucumber mosaic virus satellite RNA at novel secondary structures. Journal of Virology 81:9142-9151 (2007)

17. Yiyue Zhang, Chengwei Yang, Yin Li, Nuoyan Zheng, Hao Chen, Qingzhen Zhao, Ting Gao, Huishan Guo, and Qi Xie. SDIR1 Is a RING Finger E3 Ligase That Positively Regulates Stress-Reponsive Abscicic Acid Signaling in Arabidopsis. The Plant Cell 19:1912-1929 (2007)

16. Qi Xie and Hui-Shan Guo. Systemic antiviral silencing in plants. Virus research 118:1-6 (2006) (Review)

15. Cheng-Guo Duan, Chun-Han Wang and Hui-Shan Guo. Regulation of MicroRNA on plant development and viral infection. Chinese Science Bulletin 51:269-278 (2006) (Review)

14. Hui-Shan Guo, Qi Xie, Ji-Feng Fei and Nam-Hai Chua. microRNA directs mRNA cleagage of the transcription factor NAC1 to desensitize auxin signals for Arabidopsis lateral root development. The Plant Cell 17:1376-1386 (2005).

13. Hui-Shan Guo, Jifeng Fei, Qi Xie and Nam-Hai Chua. A chemical-regulated inducible RNAi system in plants. The Plant Journal 34:383-392 (2003).

12. Carmen Simon-Mateo, Juan Jose Lopez-Maya, Hui-Shan Guo, Elena Gonzalez and Juan Antonio Garcia. Suppressor activity of potyviral and cucumoviral infections in potyvirus-induced transgene silencing. Journal of General Virology 84:2877-2883 (2003).

11. Qi Xie, Hui-Shan Guo, Geza Dallman, Shengyun Feng, Allan M. Weissman and Nam-Hai Chua SINAT5, aRING E3 ubiquitin protein ligase, promotes post-translational degradation of NAC1 to attenuate auxin signals. Nature 419:167-170 (2002).

10. Hui-Shan Guo and ShouWei Ding A viral protein inhibits the long range signaling activity of the gene silencing signal. The EMBO Journal 21:398-407 (2002).

9. P.Lucy*, Hui-Shan Guo*, Wan-Xiang and Shou-Wei Ding Suppression of post-transcriptional gene silencing by a plant virual protein localized in the nucleus. The EMBO Journal 19:1672-1680 (2000). * contributed equally to this work *

8. Hong-Wei Li, Andrew P.Lucy, Hui-Shan Guo Wan-Xiang Li, Liang-Hui Ji, Sek-Man Wong and Shou-Wei Ding Strong host resistance targeted against a viral suppressor of the plant gene silencing defence mechanism. The EMBO Journal 18:2683-2691 (1999).

7. Hui-Shan Guo, Juan Jose Lopez-Moya and Juan Antonio García Mitotic stability of infection-induced resistance to plum pox potyvirus associated with transgene silencing and DNA methylation. Mol. Plant-Microbe Interact. 12:103-111 (1999).

6. Hui-Shan Guo, Juan Jose Lopez-Moya, Juan Antonio García Susceptibility to recombination rearrangements of a chimeric plum pox potyvirus genome after insertion of a foreign gene. Virus Research 57:183-195 (1998).

5. Hui-Shan Guo, Maria Teresa cervera and Juan Antonio García. Plum pox potyvirus resistance associated to transgene silencing that can be stabilized after different number of plant generations. Gene 206:263-272 (1998).

4. Andrés Fernández, Hui-Shan Huo , Pilar Sáenz, Laureano Simón, Gomez de Cedron M. and Juan Antonio García The motif V of plum pox potyvirus CI RNA helicase is involved in NTP hydrolysis and is essential for virus RNA replication. Nucleic Acids Research 15:4474-80 (1997).

3. Laureano Simón, Hui-Shan Guo and Juan Antonio García. Cap-indeperdent leaky scanning as the mechanism of translation initiation of a plant viral genomic RNA. Journal of General Virology 78:2691-2699 (1997).

2. Laureano Simón, Hui-Shan Guo and Juan Antonio García .Long sequences in the 5’ noncoding region of plum pox virus are not necessary for viral infectivity but contribute to viral competitiveness and pathogenesis. Virology 233:157-162 (1997).

1. Hui-Shan Guo and Juan Antonio García. Delayed resistance to plum poxpotyvirus mediated by a mutated RNA replicase gene: Involvement of a gene-silencing mechanism. Mol. Plant-Microbe Interact. 10:160-170 (1997).
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指导学生已指导学生

应晓宝 博士研究生 071007-遗传学 优秀毕业生 获2010年度所长奖学金一等奖

段成国 博士研究生 071007-遗传学

杜全生 博士研究生 071007-遗传学 优秀学生干部

何祥凤 博士研究生 071007-遗传学 获2009中科院地奥奖学金一等奖

李焱 硕士研究生 071007-遗传学

现指导学生

周邦军 博士研究生 071007-遗传学

冯镭 博士研究生 071007-遗传学

朱慧 博士研究生 071007-遗传学

徐宁 博士研究生 071007-遗传学

房媛媛 博士研究生 071007-遗传学

汪晶晶 硕士研究生 071007-遗传学

赵盼 博士研究生 071007-遗传学

赵建华 博士研究生 071007-遗传学

陈中祺 硕士研究生 071007-遗传学

张涛 博士研究生 071007-遗传学

赵云龙 博士研究生 071007-遗传学

杨丽萍 博士后

工作人员董 丽　助理研究员 ldongldong@yahoo.com.cn
段成国　助理研究员 chgduan@163.com
房媛媛　助理研究员 fangyy@im.ac.cn