1、招生信息2、教育背景3、专利与奖励4、出版信息5、指导学生6、工作经历
基本信息
黄文林 男 博导 微生物研究所
电子邮件： wl_huang@hotmail.com
联系电话： **/**
手机号码：
通信地址： 北京朝阳区北辰西路一号院三号楼中国科学院微生物研究所A428
邮政编码： 100101


研究领域病毒-宿主相互作用与生物治疗

一、病毒学研究
1）H5N1型禽流感病毒致病机制的研究
进行禽流感NS1、NS2基因与宿主细胞大分子相互作用研究，以揭示引起不同种属细胞炎性因子激活关键分子的过程及相互作用机制。对禽流感病毒感染细胞后NS1基因在细胞内聚集的相关生物学特征，NS2基因在不同细胞中对重要基因调节的信号网络的研究。
2）基于小环DNA载体的病毒疫苗研究
利用小环的低免疫原性及安全性，进行非病毒载体小环DNA的HIV、HPV等病毒疫苗方面的研究。并对其在真核细胞表达特性，免疫原性及受体-配体靶向性等方面进行研究。
3）EBV病毒致瘤机制及疫苗方面的研究
研究EBV核抗原EBNA1蛋白参与p53途径调节的机制。另外，鉴于EBNA1广泛表达于各种EBV相关的肿瘤中，研究更为安全有效的针对加强CTL反应的疫苗。
4）ADAR1的抗病毒机理研究
ADAR1在细胞内可以特异性的编辑双链RNA分子,从而产生抗病毒效应。研究选用流感病毒、狂犬病毒等对人类社会危害较大的RNA病毒作为研究对象，主要研究细胞内ADAR1的表达量是否会影响RNA病毒在宿主细胞内的复制作用，并深入探讨ADAR1的抗病毒机理。
二、肿瘤学研究
1）靶向性纳米材料介导的抗肿瘤研究
药物纳米载体具有高度靶向、药物控制释放、提高难溶药物的溶解率和吸收率等优点，因此我们致力于构建智能性纳米，使其能够定向高效的杀伤特定的肿瘤细胞，减少对非特异组织的杀伤。
2）受体酪氨酸激酶抑制剂的抗肿瘤研究酪氨酸激酶抑制剂(TKIs)的抗肿瘤作用机制是通过以抑制肿瘤细胞的损伤修复、使细胞分裂阻滞在G1期、诱导和维持细胞凋亡、抗新生血管形成等途径实现的。TKIs还可通过下调肿瘤细胞的血管生成因子以及抑制EGFR对肿瘤血管内皮细胞的信号传导,EGFR和血管内皮生长因子受体(VEGFR)两种信号传导通路的“交叉对话”,为临床同时抑制这两种传导通路提供了合理的依据。

招生信息

招生专业071010-生物化学与分子生物学

招生方向病毒与宿主之间相互作用的分子机制
教育背景1988-1994年 普林斯顿大学分子生物学博士
专利与奖励

奖励信息1.专利“一种人血管内皮细胞生长抑制因子的重组病毒” 荣获十二届中国专利奖金奖
2.《分子病毒学》课程获2008年中国科学院教学成果奖二等奖
3. 荣获2008年国务院特殊津贴
4. 肿瘤基因治疗的基础和关键技术研究荣获广东省科技进步一等奖
5. 肿瘤基因治疗的基础和关键技术研究荣获中华科学医学奖三等奖
6. 肿瘤基因治疗的基础研究荣获自然科学一等奖

专利成果专利情况：
1 Assay method for determining Product’s R’s effect on adenovirus infection of HeLa cells, US patent:**
2 黄文林。一种人血管内皮细胞生长抑制因子的重组病毒。中国发明专利（专利号：ZL01 1 27894.3）
3 黄文林，等。一种喹淋衍生物的制备方法及用作制备治疗肿瘤疾病药物的应用。国际发明专利（申请号：PCT/CN2005/000660）
4 黄文林，等。一种酪氨酸激酶抑制剂、其制备方法及作为抗肿瘤药物的应用。国际发明专利（申请号：PCT/CN2005/000661）
5 黄文林，等。一种作为抗肿瘤药物的喹淋衍生物及其制备方法。国际发明专利（申请号：PCT/CN2005/000663）
6 黄文林，等。一种作为抗肿瘤药物应用的酪氨酸激酶抑制剂及其制备方法。国际发明专利（申请号：PCT/CN2005/000662）
7 黄文林，等。腺病毒载体SARS疫苗及其制备方法，以及相关冠状病毒S基因在制备疫苗中的应用。国际发明专利（PCT/CN2004/000505）、中国发明专利（申请号：**.7）
8 黄文林，等。重组人γ-干扰素腺病毒（Ad-rhIFNγ）以及该产物的制备方法和在制备抗病毒药物中的应用。国际发明专利（PCT/CN2004/00427）、中国发明专利（申请号：**.6）
9 黄文林，等。一种酪氨酸激酶抑制剂及其制备方法和用途。中国发明专利（申请号：**.8）
10 黄文林，等。Assay method for determining Product R’s effect on adenovirus infection of hela cells。美国专利（US patent: **）
11曾益新、黄文林，等。SARS核酸疫苗及其制备方法，以及相关冠状病毒S基因在制备疫苗中的应用。中国发明专利（申请号：**.5）
12.黄文林等，HIV-gag 基因遗传密码子优化及艾滋病疫苗的制备方法与应用 **8.X
13.黄文林等，一种新型组织特异性小环载体IFNγ基因治疗药物，中国专利申请号：**2.7
14.黄文林等，一种喹唑啉生物的制备方法及用作制备治疗肿瘤疾病药物的应用，2010年获得授权，中国专利授权号：ZL**9.8
15.黄文林等，一种喹唑啉生物的制备方法及用作制备治疗肿瘤疾病药物的应用，2010年获得授权，美国专利授权号：US **.

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出版信息

发表论文1. Hongli Li, Su Li, Xubin Lin, Ye Cao, Wenqi Jiang, Ranyi Liu, Xiaofeng Zhu, Musheng Zeng, Zhongzhen Guan, Wenlin Huang. 2007. Pharmacokinetic and Pharmacodynamic Study of Intratumoral Injection an Adenoviral Encoding Endostatin in Patients with Advanced Cancer. Gene Therapy. 2008 Feb;15(4):247-56.
2. Peng Zhao, Jiangxue Wu, Hongli Li, Xia Xiao, Fajun Xie, Yinghui Zhu, Wenlin Huang*. E10A, an adenovirus carrying human endostatin gene, in combination with docetaxel treatment inhibits prostate cancer growth and metastases. Journal of Cellular and Molecular Medicine. 2007 .Accepted.
3. Liang Z, Wu J, Huang J, Tan W, Ke M, Liu R, Huang B, Xiao X, Zhao P, Wenlin Huang*. Bioactivity and stability analysis of endostatin purified from fermentation supernatant of 293 cells transfected with Ad/rhEndo. Protein Expr Purif. 2007 Dec; 56(2):205-11.
4. Guan Z, Wang XR, Zhu XF, Huang XF, Xu J, Wang LH, Wan XB, Long ZJ, Liu JN, Feng GK, Huang W, Zeng YX, Chen FJ, Liu Q Aurora-A, a negative prognostic marker, increases migration and decreases radiosensitivity in cancer cells. Cancer Res. 2007 Nov 1;67(21): 10436-44.
5. Xia Xiao, Jiangxue Wu, Jinling Zhou, Peng Zhao, Xiaofeng Zhu, Qiang Liu, Musheng Zeng, Wenlin Huang. 2007. Induction of cell Cycle Arrest and Apoptosis in Human Nasopharyngeal Carcinoma Cells by ZD6474, an Inhibitor of VEGFR Tyrosine Kinase with Additional Activity against EGFR Tyrosine Kinase. International Journal of cancer. 2007 Nov 1;121(9):2095- 104
6. Gang Xue, Ran-yi Liu, Yan Li, Ying Cheng, Zhi-hui Liang, Jiang-xue Wu, Mu-sheng Zeng, Fu-zhou Tian and Wenlin Huang*. 2007. Dendritic cells modified with 6Ckine/IFNγ fusion gene induce specific cytotoxic T lymphocytes in vitro. Cancer Immunology, Immunotherapy. 2007 Nov;56(11):1831-43.
7. Bijun Huang, Ranyi Liu, Jialing Huang, Zhihui Liang, George Fu Gao, Jiangxue Wu, Wenlin Huang*. 2007. Long-term Toxicity Studies in canine of E10A, an Adenoviral Vector for Human Endostatin Gene. Human Gene Therapy. 2007 Mar;18(3):207-21
8. Peng Zhao, Yinghui Zhu, Jiangxue Wu*, Ranyi Liu, Xiuyun Zhu, Xia Xiao,Hongli Li, Bijun Huang, Fajun Xie, Jiemin Chen, Miaola Ke, Wenlin Huang*. 2007. Adenovirus-mediated delivery of human IFNγ gene inhibits prostate cancer growth. Life Sciences. 81:695-701
9. Li J, Zeng XH, Mo HY, Rolén U, Gao YF, Zhang XS, Chen QY, Zhang L, Zeng MS, Li MZ, Huang WL, Wang XN, Zeng YX, Masucci MG.Functional Inactivation of EBV-Specific T-Lymphocytes in Nasopharyngeal Carcinoma: Implications for Tumor Immunotherapy.PLoS ONE. 2007 Nov 7;2(11):e1122
10. Li J, Zhang XS, Xie D, Deng HX, Gao YF, Chen QY, Huang WL, Masucci MG, Zeng YX.Expression of Immune-Related Molecules in Primary EBV Positive Chinese Nasopharyngeal Carcinoma: Associated with Latent Membrane Protein 1 (LMP1) Expression.Cancer Biol Ther. 2007 Oct 13;6(12)
11. Huang J, Wang F, Argyris E, Chen K, Liang Z, Tian H, Huang W, Squires K, Verlinghieri G, Zhang H.Cellular microRNAs contribute to HIV-1 latency in resting primary CD4+ T lymphocytes. Nat Med. 2007 Oct;13(10):1241-7. Epub 2007 Sep 30.
12. Wu J, Jin YJ, Calaf GM, Huang WL, Yin Y. PAC1 is a direct transcription target of E2F-1 in apoptotic signaling.Oncogene. 2007 Oct 4;26(45):6526-35. Epub 2007 Apr 30.
13. Song LB, Liao WT, Mai HQ, Zhang HZ, Zhang L, Li MZ, Hou JH, Fu LW, Huang WL, Zeng YX, Zeng MS. The clinical significance of twist expression in nasopharyngeal carcinoma.Cancer Lett. 2006 Oct 28;242(2):258-65.
14.Wu J, Xiao X, Zhao P, Xue G, Zhu Y, Zhu X, Zheng L, Zeng Y, Huang W. Minicircle-IFNgamma induces antiproliferative and antitumoral effects in human nasopharyngeal carcinoma.Clin Cancer Res. 2006 Aug 1;12(15):4702-13
15. Song LB, Zeng MS, Liao WT, Zhang L, Mo HY, Liu WL, Shao JY, Wu QL, Li MZ, Xia YF, Fu LW, Huang WL, Dimri GP, Band V, Zeng YX. Bmi-1 is a novel molecular marker of nasopharyngeal carcinoma progression and immortalizes primary human nasopharyngeal epithelial cells. Cancer Res. 2006 Jun 15;66(12):6225-32
16. Zhou JM, Zhu XF, Lu YJ, Deng R, Huang ZS, Mei YP, Wang Y, Huang WL, Liu ZC, Gu LQ, Zeng YX.Senescence and telomere shortening induced by novel potent G-quadruplex interactive agents, quindoline derivatives, in human cancer cell lines. Oncogene. 2006 Jan 26;25(4):503-11
17. Huang JL, Huang J, Duan ZH, Wei J, Min J, Luo XH, Li JG, Tan WP, Wu LZ, Liu RY, Li Y, Shao J, Huang BJ, Zeng YX, Huang W. Th2 predominance and CD8+ memory T cell depletion in patients with severe acute respiratory syndrome.Microbes Infect. 2005 Mar;7(3):427-36. Epub 2005 Feb 24.
18. Li L, Liu RY, Huang JL, Liu QC, Li Y, Wu PH, Zeng YX, Huang W. Adenovirus-mediated intra-tumoral delivery of the endostatin gene inhibits tumor growth in nasopharyngeal carcinoma. Int J Cancer, 118(8):2064-71, 2006
19. XF Liu, YF Xia…..W Huang, The Effect of p21 Antisense Oligodeoxynucleotides on the Radiosensitivity of Nasopharyngeal Carcinoma Cells with Normal p53 Function, Cell Biol. Intl, 2006, 30(3):287-7
20. JC Xia, DS Weng….W Huang, Loss of Heterozygosity Analysis of a Candidate Gastric Carcinoma Tumor Supressor Locus at 7q31, Cancer Genet Cyto Genet, 2006, 166(2):166-72
21. LB Song, MS Zeng,....W Huang, Bmi-1 is a Novel Molecular Marker of Nasopharyngeal Carcinoma Progression and Immortalizes Primary Human Nasopharygeal Epithelial Cells.Cancer Research, 2006, 66(12):6225-32
22. J-M Zhou….W-L Huang, Senescence and Telomere Shortening Induced by Novel Potent G-quadruplex Interactive Agents, Quindoline Derivatives, in Human Cancer Cell Lines, Oncogens, 2006, 25, 503-511
23. XiaoQing Li,….W. Huang, In vitro Effect of Adenovirus-mediated Human Gamma Interferon Gene Transfer into Human Mesenchymal Stem Cells for Chronic Myelogenously Leukemia, Hematological Oncology, 2006
24. Xiao Lin, Tan W., Huang W., The Biological Function of Tbet on the Microphage(Raw246.7) of Mice. Chinese Journal of Cancer.2006
25. Xue Gang…Huang WL, Construction of Recombinant Adenovirus Carrying Human IFN-γ Gene by in Vitro Ligation and its Expression, Chinese Journal of Cancer, 25(6):771-774, 2006
26. Xiao,Lin...Huang, W. GATA-3 transfusion in T Cell, Foreign Medical Sciences Blood Transfusion and Hematology,2005, 28:314-317
27. Liu, Ranyi…Huang, W. Construction of Adenovirus Harboring N-fragment of SARS-CoV, Journal of Sun Yat-sen University (Medical Sciences), 2005, 26:32-34
28.Liu, Ran-yi…Huang, W. Adenoviral Expression of a Truncated S1 Subunit of SARS-CoV Spike Protein Results in Specific Humoral Immune Responses Against SARS-CoV in Rats, Virus Research, 2005:24-31
29. Tan,Wei-ping…Huang,W. Construction of Murine T-bet cDNA Recombinant Adenovirus Vector and its Identification. Journal of Sun Yat-sen University(Medical Sciences), 2004,25:546-553
30. He, Gou-An….Huang, W. Dynamic Distribution and Expression in vivo of Human Endostatin Gene Deliveried by Adenoviral Vector, Life Science, 77:1331-1340,2005
31. Zhang, Yanlin…. Huang, Wenlin, etc. Evaluation of The Titer of Adenovirus with Immunological Method. Chi. J. Pathophysiology, 2005,21(4):830-832
Huang, Jialin….Huang, W. Th2 Predominance and CD8+ Memory T Cell Depletion in Patients with Severe Acute Respiratory Syndrome, Microbe and Infections, (7)427-436,2005
32. Li, Xiaoqing, Huang, Wenlin. In vitro Anti-leukeemic Effects of Bone Marrow Stromal Cells Modified with Ad/hIFN-r. Journal of Guangdong College of Pharmacy, 21:307-311, 2005
33. Huang,Jialing, Huang, Wenlin. Construction of Hepatocellular Carcinoma-Specific Eukarotypic Expression Vector Carrying Double Suicide Genes, Journal of Sun Yat-sen University (Medical Sciences),26(2):142-145, 2005
34. Wu, Jiangxue and Huang, Wenlin. Research Advancement of Endogenous Angiogenesis Inhibitors, Chinese Journal of Cancer, 24(3):376-384, 2005
35 .Huang, W., S.J. Flint, Unusual Properties of Adenovirus E2E Transcription by RNA Polymerase III, Journal of Virology, 77, 4015-4024, 2003
36. Huang, W.; Flint, S.J. The Tripartite Leader Sequence of Subgroup C Adenovirus Major Late mRNA Can Increase the Efficiency of mRNA Export. Journal of Virology, 72, 225-235, 1998
37.Yang, U.C.; Huang, W.; Flint, S. J. Messenger RNA Export Correlates with Activation of Transcription in Human Subgroup C Adenovirus-Infected Cells. J. of Virology, 70, 4071-4080, 1996
38. Huang , W.; Pruzan, R.; Flint, S.J. In Vivo Transcription from the Adenovirus E2E Promoter by RNA Polymerase III. Proc. Natl. Acad. Sci., USA 91, 1265-1269, 1994
39. Huang, W., etc. Inhibition of Adenovirus E1A Expression in HeLa Cells by Product R, a Peptide Nucleic Acid Immunomodulator. Journal of Infectious Diseases, 31-1, 328-231. 2001
40. Huang, W., etc. A Peptide Nucleic Acid Complex, Interferes with Expression of the E7 Oncoprotein of Human Papilloma Virus-18 in HeLa Cells. Journal of Infectious Diseases, 31-1, 220-227. 2000
41. An, H…Huang, W., etc. Construction of p53 in Adenovirus Vector. Bulletin of Fourth Military Medical University, 21(2):220-222, 2000
42. Huang, W., etc. Construction of Inducible Adenovirus Expression Vector, Journal of Medicine Chin. 12:73-80, 1998
43. Huang, W., Daiming Fang, Distribution of mRNA Expression of The Tyrosine Kinase C Receptor in Rat Spinal Cord by in Situ Hybridization. Biochemistry, 10.30;(S:S):344, 1997
44. Liu, Y…. Huang, W. etc., Application of Recombinant adenovirus for in vivo Gene Delivery to Spinal Cord. Brain Res, 768(1-2): 19-29, 1997
45. Huang, W.; Flint, S.J. The Tripartite Leader Sequence of Subgroup C Adenovirus Major Late mRNA Can Increase the Efficiency of mRNA Export. Journal of Virology, 72, 225-235, 1998
46. Yang, U.C.; Huang, W.; Flint, S. J. Messenger RNA Export Correlates with Activation of Transcription in Human Subgroup C Adenovirus-Infected Cells. J. of Virology, 70, 4071-4080, 1996
47. Huang, W.; Pruzan, R.; Flint, S.J. In Vivo Transcription from the Adenovirus E2E Promoter by RNA Polymerase III. Proc. Natl. Acad. Sci., USA 91, 1265-1269, 1994
Pruzan, R; Huang, W.; Chatterjee, P.K.; Flint, S.J. RNA Polymerase II Transcription of the Adenovirus 2 E2E Promoter. DNA Virus Conference in Cold Spring Harbor Lab, Aug. 12-16, 1992
48. Li, X.; Huang, W.; Flint, S.J. The Downstream Regulatory Sequence of the Adenovirus Type 2 Major Late Promoter Is Functionally Reductant. Journal of Virology, 66, 5685-5690, 1992
49. Huang, W.; etc., A Study on Screening of Cancers by Using Extract of Human Placenta. Health Care Journal, 6, 147-148, 1990
50. Huang, W.; etc., Inhibition of Porcine Spleen Interferon to Human Hemorrhagic Virus on Tissue Culture. Journal of Biopharmacia, 3, 107-110, 1989
50. Huang, W.; etc., Serological Study on the RHDV. Virologica Sinica, 1 ,90-95, 1989
52. Huang, W.; etc., Antigenic Relationship Between Porcine Spleen Cell Interferon and 52.Human Leukocyte Interferon. Virologica Sinica, 2, 193-198, 1989
53. Huang, W.; etc., Some Antigenic Relationship between Porcine Spleen IFN and HuIFN by McAb of HuIFN-alpha. Biochemistry and Biophysics Sinica 15, 231-233, 1988
54. Huang, W.; etc., Separation of the Porcine Spleen Interferon (PsIFN) on Fast Performance Liquid Chromatography (FPLC). Progress in Biochemistry and Biophysics, 15, 231-233, 1988
55. Huang, W.; etc., Research on Porcine Spleen Interferon. Second SCBA International Symposium at University of California, Berkeley, June 27, 1988
56. Huang, W.; etc., Approval on the Identification and Classification of Rabbit Hemorrhagic Disease Virus in Wuhan Virologica Sinica, 1, 7,1988
57. Huang, W.; etc., Study on the Heterogenic of Some Animal IFNs. Journal of Biochemical Pharmaceutic, 3, 43-49, 1987
58. Huang, W.; etc., Some Problems of Animal IFNs Heterogenic. Biochemistry of the Life, 7, 9-10,1987
59. Huang, W.; etc., Production and Antiviral Effects of a Porcine Leukocyte IFN. Acta Virologica Sinica, 5, 57-62,1987
60. Zhang, G., Huang, W. Serological Study on NPV Protein and Virion from the NPV of Cotton Bollom. Acta Microbiological Sinica, 25, 262-269,1985
61. Huang, W.; etc., Serological Detection of Some Animal Inoculated with Nuclear Polyhedron Virus (NPV) of Cotton Bollom. Acta Virological Sinica, 3, 159-163,1983


发表著作1. 《信号转导》 主编黄文林，人民卫生出版社，2005.7
2. 《分子病毒学》（第二版） 主编黄文林，人民卫生出版社，2006.12
3. 《肿瘤分子靶向治疗》 主编黄文林，人民卫生出版社，2009.5
3. 《黑色素瘤基础与临床》 主编张晓实，陈映波，黄文林，人民卫生出版社
4. 《肿瘤学》 黄文林参编，卫生部八年制教材，人民卫生出版社，2010
5. 《肿瘤生物治疗》黄文林参编
6. 《基因转录的调控》黄文林参编
指导学生已指导学生

张珍珠 硕士研究生 071010-生物化学与分子生物学

刘鹤 硕士研究生 071010-生物化学与分子生物学

张超 硕士研究生 071010-生物化学与分子生物学

王清涛 硕士研究生 071010-生物化学与分子生物学

现指导学生

姜威 博士研究生 071010-生物化学与分子生物学

工作经历1977-1988年 武汉病毒中心助理研究员
1988-1996年 普林斯顿大学分子生物学博士，助理研究员
1996-1997年 美国Allegheny大学副教授
1998-2001年 美国普林斯顿大学分子生物学系项目负责人，纽约高等病毒研究所资深科学家
2001-2005年 中山大学肿瘤防治中心教授、博士生导师，《癌症》副主编
2006-至今 中国科学院微生物研究所研究员、博士生导师， 香港中文大学荣誉教授，旅美科协生物医药学会会长，《Cancer Biology》主编。