姓名：黄坚 性别：男 职称：研究员、教授 招生院所：附属友谊医院 招生类型：科学学位(博) 科学学位(硕) 招生专业：肿瘤学(博) 肿瘤学(硕) 研究方向名称：消化系统肿瘤发病机理及诊断标志物筛选，遗传代谢性肝病基因突变及其致病机制 电子信箱：hjlq123@yeah.net


社会任职
1. 中国研究型医院学会分子诊断医学专业委员会遗传代谢性疾病学组委员
2. 国家自然科学基金、北京市自然科学基金等项目评审专家
3. 中华医学会微生物学和免疫学分会免疫诊断学组委员
4. 北京医学会肝病学分会肝癌学组委员
5. 中国研究型医院学会分子诊断医学专业委员会委员
6. 中国研究型医院学会生物标志物专业委员会委员
7. 国家药品标准物质委员会医疗器械（体外诊断试剂）分委会委员
8. 中国医药生物技术协会生物诊断技术分会委员
个人简历
1. 198409-198807:江西大学(现南昌大学)生物系 本科学习
2. 198808-199008:江西省教育学院生物系 助教
3. 199009-199212:江西OAI中德联合研究院 攻读硕士学位
4. 199303-200010:江西江中制药集团北京江中药物研究所 工程师
5. 199709-200007:军事医学科学院第11所全军基因工程重点实验室 攻读博士学位
6. 200011-200103:江西江中制药集团北京江中药物研究所 高级工程师
7. 200104-200606:军事医学科学院二所生物技术中心/深圳益生堂生物企业有限公司 高级工程师
8. 200606-200901:WHO国际肿瘤研究中心（International Agency for Research on Cancer, Lyon, France）分子病理室 博士后
9. 200903-200906:军事医学科学院二所生物技术中心 高级工程师
10. 200906-200910:首都医科大学附属北京友谊医院肝病中心/实验中心 副研究员
11. 200911-:首都医科大学附属北京友谊医院科研实验中心 研究员，教授
重要学术论文
1. Zhou D#, Qi S#, Zhang W, Wu L, Xu A, Li X, Zhang B, Li Y, Jia S, Wang H, Jia J, Ou X, Huang J*, You H*.Insertion of LINE-1 Retrotransposon Inducing Exon Inversion Causes a Rotor Syndrome Phenotype.. Front Genet. 2020,11:77:doi: 10.3389/fgene.2020.00077.SCI
2. Wu L#, Li Y#, Song Y, Zhou D, Jia S, Xu A, Zhang W, You H, Jia J, Huang J*, Ou X*.A recurrent ABCC2 p.G693R mutation resulting in loss of function of MRP2 and hyperbilirubinemia in Dubin-Johnson syndrome in China.. Orphanet J Rare Dis. 2020,15(1):74.SCI
3. Li X#, Zhang W#, Zhou D#, Lv T, Xu A, Wang H, Zhao X, Zhang B, Li Y, Jia S, Wang Y, Wang X, Wu Z, Duan W, Wang Q, Nan Y, Shang J, Jiang W, Chen Y, Zheng S, Liu M, Sun L, You H, Jia J*, Ou X*, Huang J*.Complex ATP7B mutation patterns in Wilson disease and evaluation of a yeast model for functional analysis of variants.. Hum Mutat. 2019,40(5):552-565.SCI
4. Zhang W#, Xu A#, Li Y#, Zhao S, Zhou D, Wu L, Zhang B, Zhao X, Wang Y, Wang X, Duan W, Wang Q, Nan Y, You H, Jia J*, Ou X*, Huang J*.A novel SLC40A1 p.Y333H mutation with gain of function of ferroportin: A recurrent cause of haemochromatosis in China.. Liver Int. 2019,39(6):1120-1127.SCI
5. Chen H#, Zhang Y#, Li S#, Li N, Chen Y, Zhang B, Qu C, Ding H, Huang J*, Dai M*.Direct comparison of five serum biomarkers in early diagnosis of hepatocellular carcinoma.. Cancer Manag Res. 2018,10:1947-1958.SCI
6. Wu L, Zhang W, Jia S, Zhao X, Zhou D, Xu A, Duan W, Wu Z, Li H, Zheng S, Nan Y, Jia J, Huang J*, Ou X*.Mutation analysis of the ABCC2 gene in Chinese patients with Dubin-Johnson syndrome.. Exp Ther Med. 2018,16(5):4201-4206.SCI
7. Xu A*, Li Y, Zhao W, Hou F, Li X, Sun L, Chen W, Yang A, Wu S, Zhang B, Yao J, Wang H, Huang J*.PHP14 regulates hepatic stellate cells migration in liver fibrosis via mediating TGF-β1 signaling to PI3Kγ/AKT/Rac1 pathway.. J Mol Med . 2018,96(2):119-133.SCI
8. Li X, Xu A, Li H, Zhang B, Cao B*, Huang J*.Novel role of apatinib as a multi-target RTK inhibitor in the direct suppression of hepatocellular carcinoma cells. Biochim Biophys Acta. 2018,1864(5 Pt A):1693-1701.SCI
9. Lv T#, Zhang W#, Xu A#, Li Y, Zhou D, Zhang B, Li X, Zhao X, Wang Y, Wang X, Duan W, Wang Q, Xu H, Zheng J, Zhao R, Zhu L, Dong Y, Lu L, Chen Y, Long J, Zheng S, Wang W, You H, Jia J*, Ou X*, Huang J*..Non-HFE mutations in haemochromatosis in China: combination of heterozygous mutations involving HJV signal peptide variants. J Med Genet. 2018,55（10）:650-660.SCI
10. Xu A*, Li Y, Chen W, Li X, Zhang W, Li S, Wu L, Wu Z, Zhang B, Ou X, Huang J*."Haemochromatotic" characteristics of the human BEL-7402 cell line.. Br J Haematol. 2018,183(2):302-306.SCI
11. Zhang N#, Zhao Z#, Long J#, Li H, Zhang B, Chen G, Li X, Lv T, Zhang W, Ou X, Xu A*, Huang J*.Molecular alterations of the NF2 gene in hepatocellular carcinoma and intrahepatic cholangiocarcinoma. Oncol Rep. 2017,38(6):3650-3658.SCI
12. Zhang W#, Lv T#, Huang J*, Ou X*.Type 4B hereditary hemochromatosis associated with a novel mutation in the SLC40A1 gene: A case report and a review of the literature.. Medicine. 2017,96(38):e8064.SCI
13. Xu A#*, Li X, Li S, Sun L, Wu S, Zhang B, Huang J*.A novel role for 14-kDa phosphohistidine phosphatase in lamellipodia formation. Cell Adh Migr. 2017,11(5-6)::488-495.SCI
14. Xu A, Lv T, Zhang B, Zhang W, Ou X, Huang J*.Development and evaluation of an unlabeled probe high-resolution melting assay for detection of ATP7B mutations in Wilson's disease. J Clin Lab Anal. 2017,31(4): doi: 10.1002/jcla.22064.SCI
15. 1.He X, Hong Y,Wang X, Zhang X, Long J, Li H, Zhang B, Chen S, Liu Q, Li H, Wang X, Ou X, Huang J*.Identification and clinical significance of elevated level of serum aminoacylase-1 autoantibody in patients with hepatitis B virus-related liver cirrhosis. Mol Med Rep. 2016,14(5):4255-4262.SCI
16. Lv T,li X,Zhang W, Zhao X, Ou X, Huang J*.Recent advance in the molecular genetics of Wilson’s disease and hereditary hemochromatosis. Eur J Med Genet. 2016,59(10):532-9.SCI
17. Yu Hong#, Jiang Long#, Hai Li#, Shuhong Chen, Qiqi Liu, Bei Zhang, Xiaomin He, Yan Wang, Hongyi Li,Yimei Li, Tao Zhang, Chenzhen Lu, Hao Yan, Minli Zhang, Qing Li, Bangwei Cao, Zhigang Bai, Jin Wang, Zhongtao Zhang,Xiaojuan Ou, Hong Ma, Jidong Jia, Hong You*, Shengqi Wang*, Jian Huang*.An Analysis of Immunoreactive Signatures in Early Stage Hepatocellular Carcinoma. EBioMedicine. 2015,2(5):438-46.SCI
18. Yan Wang,Man Li,Jiang Long,Xiao-Yan Shi,Qian Li,Jia Chen,Wei-Min Tong,Ji-Dong Jia, Jian Huang*.Clinical significance of increased expression of Nijmegen breakagesyndrome gene (NBS1) in human primary liver cancer. Hepatol Int. 2014,8（2）:250-259.SCI
19. Long J#, Wang Y#, Li M, Tong WM, Jia JD*, Huang J*.Correlation of TP53 mutations with HCV positivity in hepatocarcinogenesis: identification of a novel TP53 microindel in hepatocellular carcinoma with HCV infection.. Oncol Rep. 2013,30(1):119-24.SCI
20. 黄坚*，赵艳平，李倩，张俊霞，王岩，张蓓.NBS1基因SNPs与中国人群原发性肝癌的遗传易感性. 生物化学与生物物理进展 (Prog Biochem Biophys). 2012,39(7):678-686.SCI
21. Jian Huang, Jesse Pang, Takuya Watanabe, H-K Ng, Hiroko Ohgaki.Whole Genome Amplification for Array CGH using DNA Extracted from Formalin-Fixed Paraffin-Embedded Histological Sections. J MOL DIAGN. 2009,11(2):109-116.SCI
22. Jian Huang, Grotzer MA, Watanabe T, Hewer E, Pietsch T, Rutkowski S, Ohgaki H.Mutations in the Nijmegen breakage syndrome gene in medulloblastomas. CLIN CANCER RES . 2008,14(13):4053-8.SCI
主编或副主编的著作/译著
重要奖项
1. 首都医科大学附属北京友谊医院科学技术创新奖 基于基因组不稳定性的肝癌相关自身抗体分子标志物研究 二等奖 202001
2. 生物芯片技术在军事医学研究中的应用 国家科技进步奖 二等奖 200606