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姓名：王晓佳性别：女职务：
所在系：职称：副教授导师类别：博士
政治面貌：中共党员出生年月：1976.7学位：博士
电子邮件：wangxj@cau.edu.cn办公电话：62734377
个人简介：个人简介：2006年12月受聘为中国农业大学动物医学院副教授，研究方向为重要畜禽病毒性疾病的致病机制与新型防控技术。作为第一主讲人，负责全校研究生选修课《蛋白质及蛋白质技术》，第三主讲国家级精品课程《兽医寄生虫学》。2010年3月-2011年9月受聘为美国芝加哥大学微生物系访问助理教授，研究方向为疱疹病毒与宿主细胞的相互作用机理。2006年“副粘病毒入侵宿主细胞的分子机制研究”获得教育部全国“百篇”优秀博士论文。近年以第一作者或通讯作者在国际权威的PNAS（美国科学院报）、Virology（病毒学杂志）等SCI收录期刊发表论文11篇，国内核心杂志发表论文4篇，申请发明专利4项，参编著作4部。主持高等学校全国优秀博士学位论文作者专项资金、国家自然科学基金、国家十一五攻关、教育部博士点新教师基金、国家十五攻关、科技部基本科研业务费各1项，副主持国家高技术研究发展计划（863）1项。熟练掌握英语与俄语，积极推进国际合作。2008年获德意志学术中心邀请与资助，在德国汉诺威大学医学院展开3个月的国际合作研究，并受邀报告；2008年在教育部的外教师资计划资助下，邀请美国农业部的知名专家，在校内组织开展一系列学术讲座。SCI收录论文（*通讯作者）：Wang,X.J.,Patenode,C.,Roizman,B.TheUS3proteinkinaseofHSV-1cyclesbetweenthecytoplasmandnucleusandinteractswithPDCD4toblockapoptosis.ProcNatlAcadSciUSA,2011,108(35),14632-36.Wang,X.J.,*Li,C.G.,Chi,X.J.,Wang,M.CharacterisationandevaluationofantiviralrecombinantpeptidesbasedontheheptadrepeatregionsofNDVandIBVfusionglycoproteins.Virology,2011,416(1-2),65-74.Wang,X.J.*Chi,X.J.,Wang,M.StructuralcharacteristicsandantiviralactivityofmultiplepeptidesderivedfromMDVglycoproteinsBandH.VirologyJournal,2011,8,190-203.Wang,X.J.*Heat-shockprotein70isassociatedwiththeentryofMarek’sdiseasevirusintocells.ActaVirologica,2011,55,3.Wang,X.J.*Themolecularmechanismofherpesvirusmembranefusion,ProgressinBiochemistryandBiophysics,2010,37(6),583-88.(reviewarticle)Wang,X.J.andWang,M.ThemolecularmechanismofclassIIenvelopedvirusesmembranefusion,ProgressinBiochemistryandBiophysics,2007,34(7),682-86.(reviewarticle)Wang,X.J.,Bai,Y.D.,Zhang,G.Z.,Zhao,J.X.,Wang,M.,andGao,G.F.Structureandfunctionstudyofparamyxovirusfusionproteinheptadrepeatpeptides,ArchivesofBiochemistryandBiophysics,2005,436(2),316-322.Wang,X.J.,Zhang,G.Z.,Zhao,J.X.,andWang,M.InteractingdomainsoftheHNandFproteinsofparamyxovirus,ChineseScienceBulletin,2005,50(20),2231-2234.Wang,X.J.,Zhu,D.B.,Zhang,G.Z.,Bai,Y.D.,andWang,M.Constructionandexpressionofcorrelativegeneswithmembranefusionofparamyxovirus,ProgressinBiochemistryandBiophysics,2005,32(3),228-34.Wang,X.J.,Xu,Y.H.,Cole,D.K.,Lou,Z.Y.,Liu,Y.W.,Rao,Z.H.,Wang,M.,andGao,G.F.Biochemical,biophysicalandX-raycrystallographicanalysesofthefusioncoreofSendaivirusFprotein,ActaCrystallographicaSectionD,2004,60(9),1632-1635.Wang,X.J.,Zhang,W.H.,Wang,M.,andGao,G.F.TheMolecularmechanismofenvelopedvirus-cellmembranefusion,ProgressinBiochemistryandBiophysics,2004,31(6),482-491.(reviewarticle)专利：1.HR2模拟蛋白及其编码基因与应用.200610144153.（第1作者）2.NovelapplicationofCoccidian.200710064924（第2作者）3.鸡球虫抗药性检测方法及其检测试剂盒.200910080180（第2作者）4.具有抑制病毒混合感染活性的多肽.2010101218301.（第1作者）负责课题：1.马立克氏病毒与宿主细胞膜融合的分子机制与抗病毒抑制研究，高等学校全国优秀博士学位论文作者专项资金，教育部，200769，2007-2012，主持2.球虫抗药性检测与控制关键技术研究，2006BAK02A19-4，国家十一五科技支撑计划，科技部，2006-2008，主持3.HR模拟蛋白的构建、结构与抗病毒功能研究，20070019068，博士点新教师基金，教育部，2008-2010，主持4.基于七肽重复区域设计的马立克氏病毒新型抑制物研究，2009JS10，教育部基本科研业务费,2010年，主持5.鸡球虫顶膜抗原（AMA）的细胞入侵作用和免疫原性研究，GSKJ070202，广东省兽医公共卫生公共实验室开放基金项目，广东省科技部，2007-2009，主持6.表达并运送禽流感病毒抗原组的新型疫苗活载体研制，2006AA02Z458，科技部“863”计划，科技部，2006-2008，副主持7.抑制新城疫与传染性支气管炎病毒混合感染的多肽作用机理，31101819，国家自然基金委，2011-2014，主持MAJORRESEARCHEXPERIENCE：2010-present,Thisprojectconcernstheherpessimplexvirus1(HSV-1)US3andapotentialant-apoptotictargetoftheenzyme.TheUS3proteinkinaseofherpessimplexvirus1playsakeyroleinblockingapoptosisinducedbyviralgeneproductsorexogenousagents.WefindthatintheyeasttwohybridsystemadomainofUS3essentialforanti-apoptoticactivityreactedwithPDCD4(ProgrammedDeathprotein4).US3interactswithPDCD4,andPDCD4isposttranslationallymodifiedininfectedcellsbothinaUS3dependentandindependentfashionandthatdepletionofPDCD4bysiRNAblockedapoptosisinducedbymutantvirus.IninfectedcellsPDCD4accumulatesinthenucleuswhereasUS3accumulatesinthecytoplasm.StudiesdesignedtoelucidatetheconvergenceoftheseproteinsledtothediscoverythatUS3proteinkinasecyclesbetweenthenucleusandcytoplasmandthatUS3retainsPDCD4ininfectedcellnuclei.PartoftheresultwaspublishedinProcNatlAcadSciUSA.2008-present,Lossescausedbymixedvirusinfectionsaremuchmoredevastatingthansinglevirusinfections.Newcastlediseasevirus(NDV)andinfectiousbronchitisvirus(IBV),seriousthreatstothepoultryindustry,cangiverisetocomplexmix-infectionswhichhinderdiagnosisandprevention.Inthisstudy,itisshownthatthenewlydesignedpeptides,whicharedevelopedbasedontheheptadrepeat-2(HR2)regionofthefusionglycoproteinsfromNDVandIBV,canshowamorepotentantiviralactivitythanthemotherHR2peptides.Experimentsconductedontheplaqueformationwithchickenembryoassaysconfirmedtheresult.ThedesignedpeptidescancompletelyinhibitsinglevirusinfectionsandmixedinfectioncausedbyNDVandIBV.Further,celltoxicityandpossibletargetsforpeptideshavebeenstudied,therebystrengtheningthenotionthatHR2isanattractivesitefortherapeuticintervention.Thepresentpaperoffersthepossibilityofdesigningarelativelybroad-spectrumclassofantiviralmix-infectioninhibitors.AChinesepatenthasbeenawarded.PartoftheresultwaspublishedinVirology.2008-present,StudyonInteractionsbetweenEnvelopeProteinsandtheirCellularReceptorsofMarek′sDiseaseVirus.ResultsfromaWesternblotanalysisofcellularproteinsforvirusreceptorsandco-immunoprecipitationsuggeststhatheatshockprotein70(HSP70)isapotentialcellularreceptorforMDVglycoproteingH.PlaqueinhibitionassaysconfirmtheinvolvementofHSP70intheearlystagesofMDVentryintochickenembryofibroblasts(CEF).ThepresentworksupportsthatHSP70isimplicatedintheMDVentryprocessbybindingtogH,andenhancestheunderstandingofmultifunctionalHSP70andtheMDVinfectionprocess.PartoftheresultwaspublishedinActaVirologica.Anotherpaperisbeingpreparedforpublication.2007-present,StudyonfunctionaldomainsanalysisofMDVgHandgB,todeterminethepeptideshomologregioninwhichvirusentrycanbeinhibited.Novelheptadrepeat(HR)peptides,modifiedbyN-4EK/C-4K,withcascadeconnectionofantiviralHRregionsfromdifferentstagesduringvirusentry,wereshowntobemorepotentantiviralinhibitors.CDandGel-filtrationandIPwereusedtofindinhibitor’starget.Theanimalexperimentsfurtherprovedthatthenovelconstructionpeptidesprovidedastableandhighlyeffectivestrategyfordevelopingnovelantiviraldrugs.AChinesepatenthasbeenawarded.PartoftheresultwaspublishedinVirologyJournal.2004-2006,StudyonHNandFGlycoproteinInteractionofParamyxovirus.TheGST-pulldownandMSandCDresultsshowedthattheglobularheaddomainofHNcouldinteractwithHR1andHR2ofFprotein.Thestudiesprovideddirectinsightintotheinteractiondomainofthetwoglycoproteinsofparamyxovirus,andconsequentlycompletedthemolecularmechanisminwhichtheHNproteinactivatestheFproteininducingmembranefusion.ResultswerepublishedinChineseScienceBulletinandProgressinBiochemistryandBiophysics.2001-present,Ph.Dthesis:MolecularMechanismofParamyxovirusEnteringHostCell.TheCDandGel-filtrationandcrystaldiffractingX-raysresultsshowedthatheptadrepeat(HR)regionsofHR1andHR2fromtheFglycoproteinofparamyxovirusesform6-helixbundle(trimer-of-hairpins),thepost-fusionconformationofthefusioncoreoftheparamyxovirus.Thevirus-cellexperimentshowedonlysolubleHR2peptidehadpotentandspecificvirus-cellfusioninhibitionactivity.Theoutcomeprovidedasolidbasisfordevelopingnovelantiviraldrugsandrecruitinganewtheoryinvirology.ResultswerepublishedinActaCrystallographicaSectionD,ArchivesofBiochemistryandBiophysics,andProgressinBiochemistryandBiophysics.