简 介

王蔚芝主要从事纳米分析检测、纳米生物医学与靶向活体分子影像研究。以亲和多肽为主要研究工具，构筑生物分子相互作用、识别、筛选和自组装体系。在针对细胞、体液、活体的精准化学测量和针对肿瘤的靶向诊疗等方面取得创新成果。近年已在SCI期刊发表论文30余篇，包括Advanced Materials，Analytical Chemistry，Biomaterials，Lab on a Chip，Theranostics，Chemical Communications等。2015年入选北京市优秀人才培养“青年拔尖”个人。2019年入选中国科学院青年创新促进会。拥有多项授权发明专利。主持国家自然科学基金项目、北京市自然科学基金项目等。

课题组长期招收具有分析化学、生物化学、纳米材料科学、有机化学、医学、机械与流体力学等相关背景的博士后、博士生及硕士生。

教育经历

主持项目

研究兴趣

1、 纳米生物医学检测与微流控分析化学

针对肿瘤等重大疾病实现早期而精准的诊断和治疗是当今生物医学领域的难题和热点。以生物活性分子多肽等作为高灵敏识别元件，通过靶标-配体之间的特异性亲和相互作用，对肿瘤标志物进行高特异、高灵敏检测。建立了微流控芯片高通量多肽筛选新方法。实现库容量为106-108的组合化学肽库微流控分选、 阳肽捕获和基质辅助激光解吸电离- 飞行时间质谱

（MALDI-TOF-MS）原位测序并与表面等离子体共振成像/荧光成像集成联用，识别、分选、测序及亲和常数分析全流程可在几小时内完成，相较于常规方法数天的筛选周期，效率显著提高。相关结果发表于Analytical Chemistry

（2014, 86: 11854）、Analytical Chemistry（2014, 86: 3703）等。

2、 多肽靶向分子探针与活体分子影像

利用新型靶向多肽分子与近红外二区荧光分子相结合实现针对肿瘤的精准定位、术中实时导航及快速排泄。首先，小分子多肽亲和力好、免疫原性低、穿透性强并易于化学修饰，是十分理想的靶向识别单元；其次，相比于常用的可见光以及近红外一区荧光成像，近红外二区（NIR-II，1000-1700 nm发射区间）生物组织光散射及自荧光效应少，生物背景低，探测深度深、时空分辨率高。尤为重要的是，小分子多肽探针可以经泌尿系统快速排泄。6小时之内，有 90%的探针可以排出体外，具有良好的生物安全性。相关研究

结果发表于 Advanced Materials（2018, 30: **）。由于在多肽分子识别领域的一系列工作，应邀撰写关于多肽分子探针的综述 Advanced Materials

（2018, 30: **）。

3、 多肽纳米生物材料精准识别与可控自组装

构成多肽分子的氨基酸结构性质多样，可通过精妙结构单元的设计和构筑实现可控超分子自组装。例如构建一种在特异识别肿瘤新生血管的同时还能智能响应肿瘤的微酸性环境的多肽纳米材料，实现活体传感与靶向运输。该多肽在肿瘤血管标记物和酸性微环境同时存在时发生特异性响应自组装，实现纳米构象变化，使穿膜和胞内输运能力提高。相关研究结果发表于 Biomaterials（2019, 188: 96）及Chemical Communications（2016, 52: 5690）等。进一步利用靶向多肽对纳米材料功能化，研究配体密度、构型及环境响应性等指标，用于活细胞及活体的载药、输运及诊疗一体化研究，相关结果发表于Analytical Chemistry（2019, 91: 7245）及Theranostics（2019, 9: 1728）等。

代表性论文

1 、 Weizhi Wang*, Zhiyuan Hu*, Targeting Peptide-based Probes for Molecular Imaging and Diagnosis. Advanced Materials 2018, 30: **.

2 、 Weizhi Wang#, Zhuoran Ma#, Shoujun Zhu, Hao Wan, Jingying Yue, Huilong Ma, Rui Ma, Qinglai Yang, Zihua Wang, Qian Li, Yixia Qian, Chunyan Yue, Yuehua Wang, Linyang Fan, Yeteng Zhong, Ying Zhou, Hongpeng Gao, Junshan Ruan, Zhiyuan Hu*,

Yongye Liang*, Hongjie Dai*. Molecular cancer imaging in the second near-infrared window using a renal-excreted NIR-II fluorophore-peptide probe. Advanced Materials 2018, 30: **.

3、 Yuehua Wang, Fei Jia, Zihua Wang, Yixia Qian, Linyang Fan, He Gong, Aiqin Luo, Jian Sun, Zhiyuan Hu, Weizhi Wang*. Boosting the theranostic effect of liposomal probes toward prominin-1 through optimized dual-site targeting. Analytical

Chemistry 2019, 91: 7245.

4 、 Yixia Qian, Yuehua Wang, Fei Jia, Zihua Wang, Chunyan Yue, Weikai Zhang, Zhiyuan Hu, Weizhi Wang*. Tumor-microenvironment controlled nanomicelles with AIE property for boosting cancer therapy and apoptosis monitoring. Biomaterials

2019, 188: 96.

5 、 Zihua Wang, Yuehua Wang, Xiangqian Jia, Qiuju Han, Yixia Qian, Qian Li, Junfeng Xiang, Qian Wang, Zhiyuan Hu and Weizhi Wang*. MMP-2-Controlled Transforming Micelles for Heterogeneic Targeting and Programmable Cancer

Therapy. Theranostics 2019, 9: 1728.

6 、 Weizhi Wang#, Zewen Wei#, Di Zhang, Huailei Ma, Zihua Wang, Xiangli Bu, Menglin Li, Leroy Hood, Lingling Geng, Christopher Lausted, Qiaojun Fang, Hao Wang*, Zhiyuan Hu*. Rapid screening of peptide probes through in situ single bead

sequencing microarray. Analytical Chemistry 2014, 86: 11854.

7 、 Weizhi Wang#, Menglin Li#, Zewen Wei, Zihua Wang, Xiangli Bu, Wenjia Lai, Shu Yang, He Gong, Hui Zheng, Yuqiao Wang, Ying Liu, Qin Li*, Qiaojun Fang*, Zhiyuan Hu*. Bimodal imprint chips for peptide screening: integration of

high-throughput sequencing by MS and affinity analyses by Surface Plasmon Resonance imaging. Analytical Chemistry 2014, 86: 3703.

8 、 Zihua Wang#, Weizhi Wang#*, Xiangli Bu, Zewen Wei, Lingling Geng, Yue Wu, Chengyan Dong, Liqiang Li, Di Zhang, Shu Yang, Fan Wang, Christopher Lausted, Leroy Hood, Zhiyuan Hu*. Microarray based screening of peptide nano probes for

HER2 positive tumor. Analytical Chemistry 2015, 87: 8367.

9、 Weizhi Wang*#, Zihua Wang#, Xiangli Bu, Ren Li, Zhiyuan Hu*. Discovering of tumor-targeting peptides using bi-functional microarray. Advanced Healthcare Materials 2015, 4: 2802.

10、 Weizhi Wang, Yanyan Huang, Yulong Jin, Yunfeng Xie, Rui Zhao*, Shaoxiang Xiong, Guoquan Liu, Yi Chen, Huimin Ma. Integrated SPPS on continuous-flow radial microfluidic chip. Lab on a Chip 2011, 11: 929.

11 、 Yixia Qian#, Weizhi Wang#*, Zihua Wang, Qiuju Han, Xiangqian Jia, Shu Yang, Zhiyuan Hu*. Switchable probes: pH-triggered and VEGFR2 targeted peptides screening through imprinting microarray. Chemical Communications 2016, 52:

5690.

12 、 Yixia Qian#, Weizhi Wang#*, Zihua Wang, Xiangqian Jia, Qiuju Han, Iman Rostami, Yuehua Wang, Zhiyuan Hu*. pH-triggered peptide self-assembly for targeting imaging and therapy toward angiogenesis with enhanced signals. ACS Applied

Materials & Interfaces 2018, 10: 7871.

13 、 Qiuju Han#, Weizhi Wang#*, Xiangqian Jia, Yixia Qian, Qian Li, Zihua Wang, Weikai Zhang, Shu Yang, Yunhong Jia*, Zhiyuan Hu*. Switchable liposomes: targeting-peptide-functionalized and pH-triggered cytoplasmic delivery. ACS Applied

Materials & Interfaces 2016, 8: 18658.

14 、 Zihua Wang#, Weizhi Wang#*, Lingling Geng, Zhiyuan Hu*. Distinguishing of tumor cell-targeting peptide ligands through a color-encoding microarray. Lab on a Chip 2015, 15: 4512.

15 、 Yuehua Wang, Zihua Wang, Yixia Qian, Linyang Fan, Chunyan Yue, Fei Jia, Jian Sun*, Zhiyuan Hu*, Weizhi Wang*. Synergetic Estrogen receptor-targeting liposome nanocarriers with anti-phagocytic property for enhanced tumors theranostics.

Journal of Materials Chemistry B, 2019, 7: 1056.

16 、 Qiuju Han, Xiangqian Jia, Yixia Qian, Zihua Wang, Shu Yang, Yunhong Jia*, Weizhi Wang*, Zhiyuan Hu*. Peptide functionalized targeting liposomes: for nanoscale drug delivery towards angiogenesis. Journal of Materials Chemistry B

2016, 4: 7087.

17 、 Xiangqian Jia#, Weizhi Wang#*, Qiuju Han, Zihua Wang, Yunhong Jia*, Zhiyuan Hu*. Micromixer based preparation of functionalized liposomesand targeting drug delivery. ACS Medicinal Chemistry Letters 2016, 7: 429.

18 、 Lingling Geng, Zihua Wang, Xiangqian Jia, Qiuju Han, Zhichu Xiang, Dan Li, Xiaoliang Yang, Di Zhang, Xiangli Bu, Weizhi Wang*, Zhiyuan Hu*, Qiaojun Fang*. HER2 targeting peptides screening and applications in tumor imaging and

drug delivery. Theranostics 2016, 6: 1261.

19 、 Hui Zheng#, Weizhi Wang#, Xiaojun Li, Zihua Wang, Leroy Hood, Christopher Lausted, Zhiyuan Hu*. Automated Teflon microfluidic peptide synthesizer. Lab on a Chip 2013, 13: 3347.

20、 Zhuoran Ma#, Hao Wan#, Weizhi Wang#, Xiaodong Zhang, Takaaki Uno, Qianglai Yang, Jingying Yue, Hongpeng Gao, Yeteng Zhong, Ye Tian, Qinchao Sun, Yongye Liang, Hongjie Dai*. A theranostic agent for cancer therapy and imaging in the

second near-infrared window. Nano Research 2019, 12: 273.